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Alright, welcome everybody.

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Today, we're pleased to have you join us

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for the National Marine Sanctuaries webinar series.

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This series is hosted by

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the NOAA Office of National Marine Sanctuaries,

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and it gives us an opportunity to connect

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with educators and other interested parties

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to provide you with expertise,

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whether educational or scientific expertise,

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as well as resources and training

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opportunities that help support

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ocean and climate literacy with your different audiences.

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So, with that, it also gives us an opportunity

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, let me get this going again,

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an opportunity to introduce you and connect you

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to our country's national marine sanctuaries.

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So, these are our underwater treasures,

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or some people like to say, underwater parks.

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We are trustees of this system

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of thirteen national marine sanctuaries

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and the Papahānaumokuākea

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Marine National monument

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and the Rose Atoll Marine National Monument.

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So, this system actually encompasses

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more than six-hundred thousand

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square miles of marine and Great Lakes treasures.

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So,  just alone in the state of California there are four,

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all the way up to Olympic coast

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in the Olympic Peninsula.

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We have a couple in the Hawaiian islands,

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all the way down to the South Pacific

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and American Samoa,

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and starting in the Gulf of Mexico,

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working all the way around

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up into Lake Huron for the

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Thunder Bay National Marine Sanctuary.

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So, this system of national marine sanctuaries

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help protect the ocean and Great Lakes.

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Now, national marine sanctuaries are

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mandated to do research

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and monitoring and resource protection,

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along with education and outreach

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of these underwater treasures,

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and the idea is to preserve them

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for future generations.

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And these areas are set aside

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because they're so special,

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either for conservation reasons

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or historical, cultural, maritime, archaeological reasons,

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recreational , aesthetics, ecological, etc.

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So, a wide variety of reasons that

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these special ocean areas

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are put aside to be considered

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national marine sanctuaries.

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We also like to call them "living classrooms"

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because these national marine sanctuaries

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are places that people like yourselves and

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your students and your visitors can

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touch, learn, and feel about these ocean treasures.

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So, a great place to immerse yourself

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and learn more about our underwater parks.

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So, with that, I'll introduce myself.

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My name is Claire Fackler.

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I'm the national education liaison for the

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NOAA Office of National Marine Sanctuaries.

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I am hosting today's webinar from my office

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in Santa Barbara, California.

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And I will be helping answer questions from attendees

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and running our poll questions, as well as

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helping with any technical issues

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or difficulties you might have.

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And of course, we always greatly appreciate feedback.

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So, if you have any suggestions on how we can continue

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to improve this webinar series and experience,

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please take two to three minutes at the end of the webinar

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when you close out to answer our evaluation survey.

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That was greatly appreciated for our team.

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And then during the presentation,

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all of you that are joining us,

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the three-hundred and twenty-nine folks

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that have registered,

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are going to be in listen-only mode.

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So, if you have a question,

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probably the easiest thing to do

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is to just type it into the question box.

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If it's an appropriate time,

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I'll interrupt our speaker and ask

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her the question, or we will do it in our

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five or ten minutes

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of Q and A at the end of today's presentation.

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If you're bold, at the very end you can raise your hand

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in the control panel, and

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that will give me an alert that

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you would like to be un-muted

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and to ask your question that way.

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So, we will be recording today's webinar presentation.

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It will be archived, you will get a follow-up email

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that lets you know where on the internet

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the archive will be housed.

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And with that, I would like to introduce

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a colleague of mine,

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who has been involved with this dungeness crab

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ocean acidification tool kit.

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Her name is Jennifer Stock, and she's a colleague

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that works for our NOAA's Cordell Bank

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National Marine Sanctuary as

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the education outreach coordinator.

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Jenny has also been sort of the lead in

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orchestrating the development

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of this dungeness crab O-A tool kit

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with our west coast education team.

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So, with that, Jenny, go ahead and

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I'll let you run a few more slides here

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for introduction.

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- Thanks, Claire, and thanks,

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everybody, for being on today.

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I just wanted to set the scene a little bit

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since we've been working on this for a while.

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Claire showed you an image of all

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the national marine sanctuaries in our program, but

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zooming in here to the west coast, we have five of these

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special places between Washington and California.

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And operationally, we are all five really unique places

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in the ocean, but the ecosystem ties us together

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to collaborate where it makes sense.

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These five national marine sanctuaries are connected,

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and go to the next slide,

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through the productive eastern boundary current,

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the California current,

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which provides incredible productivity

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along the west coast, but also supports

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a lot of the same species

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that we have between Washington and California,

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such as the great whales and sea birds

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and keystone species

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like krill and dungeness crab,

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supporting communities

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supporting culture,

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supporting economy throughout these

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states and nation-wide.

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So, with the California current,

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you can go to the next slide,

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with this incredible great productivity, we also see

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some of the highest ocean

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acidification rates in the world

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here on the west coast.

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This image shows the current and future predictions

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of PH surface waters, which makes it harder for a lot of

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organisms. So this is a shared concern amongst

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each of these five west coast

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national marine sanctuaries

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and of course many of the other partners and agencies

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that are studying the ocean.

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So, the education coordinators from each of these

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national marine sanctuaries consider this an area

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we could really work together to help bring tools

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to have more people talking about ocean acidification

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and focusing on species that are

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really iconic and valuable

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to people, valuable to ecosystems.

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So, the dungeness crab came up as a great species to

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focus on for the development of this case study.

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So, all of our national marine sanctuary education

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coordinators helped support this, and we looked to our

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other NOAA partners at NOAA fisheries and the NOAA

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ocean acidification program to

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bring together the best science

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and make sure things are accurate

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and worked very closely

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with them. So we really appreciate the input from them.

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We also are really happy to have met

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Doctor Shelly Trigg,

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who was really the lead on developing

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the tools of this kit,

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and I'm really excited to introduce Shelly today,

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who came in as "Shelly Trigg," but came out as

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"Doctor Shelly Trigg," so congratulations on that.

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Shelly is a molecular systems biologist by training.

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She obtained her Ph.D. in Biology from U.C. San Diego

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this past August.

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Her dissertation work involved mapping

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large protein networks in plants to better understand

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gene regulation and developing tools to study molecular

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networks in any species. During her graduate studies,

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Shelly became interested in applying her systems

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biology background to helping understand climate

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change effects on marine life.

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As a National Science Foundation graduate research

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fellow, she was awarded an internship opportunity

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with the NOAA Northwest Fisheries Science Center

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to investigate ocean acidification effects on

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dungness crab on the molecular level.

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And during her internship, she collaborated with

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national marine sanctuaries in preparing a

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dungeness crab ocean acidification toolkit.

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So, Shelly, I want to introduce you and thank you for

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leading the rest of the presentation today.

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And thanks, everybody else, for joining us.

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- Excellent. We pass the controls over to you, Shelly,

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and we will take off our web cameras,

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so all attention is on you.

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- Okay. Hi, everyone.

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This is my first webinar presentation,

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so I'm pretty excited.

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Can everyone see my screen?

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- (Claire Fackler speaking) It looks great.

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Maybe just once you get it in presentation mode.

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- Presentation mode. Let's see here.

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- (Claire Fackler speaking) Alright. Take it away.

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- Hey. Alright. Yeah, so like Jenny said, we've been,

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this project, the toolkit, has been, we've been working

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on it for a while, and we're very very excited to

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release it and make it available to all of you so that,

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so all of you can share this information with people.

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So, alright, let's see. So, today this is just the outline.

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I'm gonna talk about a brief

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ocean acidifaction background,

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talk about dungeness crab background,

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and then talk about the research

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that's going on at NOAA,

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stuff in the past, stuff that I worked on in the past year,

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and then stuff that's currently on-going.

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And then I will tell you about the toolkit and all the

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different pieces of it and kind of how you can use that.

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Okay, so here's our ocean acidification background.

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So, human activities like deforestation and the

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burning of fossil fuels has changed the amount of

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carbon dioxide that is in the atmosphere, with

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fossil fuel burning causing the most change.

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And when we burn fossil fuels like coal,

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oil, and natural gas

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to make energy, we are adding

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more carbon into the atmosphere.

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Okay. So, here's our first question.

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So,  our carbon dioxide emissions

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go into the atmosphere.

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But then where do they go after that?

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So, I think...

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Claire

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- (Claire Fackler speaking) Alright, let me go

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ahead and, yep, launch that poll question.

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I got caught up answering a question online.

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So, let's launch the poll.

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And so now you have a chance to vote on what

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you think is the correct response.

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Alright, voting is picking up,

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so we've got about fifty percent

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of you. The rest of you go ahead and register your vote

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on QUICKPOLL. This is practice for early November.

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Alright, I will go ahead and close the poll

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and share the results.

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So, Shelly, it looks like eighty-one percent of our

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participants feel that a quarter of it goes into the land,

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a quarter of it goes back into the ocean, and

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fifty percent stays in the atmosphere.

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- Yeah, that's really impressive because that is like

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right on the money.

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So, this is an estimate, but that is the closest estimate.

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So, good job, everyone.

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Okay. So, every year the ocean is absorbing about

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twenty-five percent of human-caused

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carbon dioxide emissions and this is what's changing

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the ocean's chemistry.

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And one way we monitor this change is through pH,

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which is a numerical scale based on the amount of

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hydrogen ions that are in the water.

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And because hydrogen ions are so tiny, and there's

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lots of them, we convert to the pH scale to make it

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easier for us to understand the change

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in these hydrogen ions.

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So, there's this mixing of carbon dioxide with water,

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which forms carbonic acid, and that leads to the

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increase in hydrogen ions

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and the decrease in the ocean pH.

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And the decrease in the ocean pH over time is

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called ocean acidification.

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Now I'm gonna switch to dungeness crabs.

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And why they're important, why we care

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about studying them.

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They're an ecologically important species.

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There's many fish species that eat them when they're

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in the larval stage.

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And like sometimes there's been some studies

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where they're cutting open the bellies of some fish

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and all they find are these crab larvae in there.

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Dungeness crab are also culturally important,

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and west coast tribal fisheries provide food, income,

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and communal activity for Native Americans.

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And the crabs are also economically important where

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the dungeness crab fishery is one of the highest

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valued U.S. fisheries, at over, bringing in over

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two-hundred million dollars annually and supporting

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the livelihood and jobs of fisherman,

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restaurant workers, etc.

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We care about what's going to happen to them

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with ocean acidification.

00:14:14.000 --> 00:14:16.720
Having ocean acidification adds this environmental

00:14:16.740 --> 00:14:20.120
stress, which can make it more challenging for

00:14:20.260 --> 00:14:21.620
animals to survive.

00:14:23.600 --> 00:14:26.760
Calcifying animals are animals that use

00:14:27.540 --> 00:14:29.680
calcium carbonate. They take calcium carbonate

00:14:29.680 --> 00:14:32.840
out of the water to help make their shells, and

00:14:33.160 --> 00:14:36.580
doing that is a process that requires energy.

00:14:38.540 --> 00:14:41.660
Now I'm gonna talk a little bit about energy

00:14:41.660 --> 00:14:45.760
and cells and hopefully not get too crazy, but okay so

00:14:46.420 --> 00:14:49.060
animal cells, like cells that we have, and cells that

00:14:49.060 --> 00:14:52.960
crabs have, this is just like a blown-up picture of a cell,

00:14:54.840 --> 00:14:58.440
consume the food that we eat and convert it into energy

00:14:58.600 --> 00:15:02.800
and then they use the energy to fuel these different

00:15:02.800 --> 00:15:05.900
processes, and other processes to make, you know,

00:15:05.900 --> 00:15:07.020
to have the animal live.

00:15:08.180 --> 00:15:12.560
For example, cells make energy to fight off

00:15:12.600 --> 00:15:17.620
infection, to grow, to sense different things, and

00:15:18.640 --> 00:15:22.760
to breathe and a lot of other processes also.

00:15:24.300 --> 00:15:29.100
But cells and the machinery that's in them that make

00:15:29.100 --> 00:15:32.600
energy are, you know, they have a capacity.

00:15:32.600 --> 00:15:37.060
They only convert so much food into energy.

00:15:42.680 --> 00:15:46.440
They need to kind of like figure

00:15:46.440 --> 00:15:50.340
out how to use the energy that they have.

00:15:50.960 --> 00:15:57.360
One thing I wanted to ask people is like

00:15:57.960 --> 00:16:00.400
out of the four different processes that I just showed

00:16:00.400 --> 00:16:02.780
a picture of, which process do you think is most

00:16:03.320 --> 00:16:06.080
important for the animal to do?

00:16:06.460 --> 00:16:08.280
And maybe which, and in the next

00:16:08.340 --> 00:16:10.160
there's gonna be a follow-up question, which

00:16:10.320 --> 00:16:12.140
process do you think is the least important?

00:16:12.540 --> 00:16:13.560
- (Claire Fackler speaking) Alright, so we've

00:16:13.560 --> 00:16:15.120
gone ahead and launched the first

00:16:15.120 --> 00:16:17.440
poll in the series of the two-part question.

00:16:17.440 --> 00:16:20.240
So which processes is the most important:

00:16:20.440 --> 00:16:23.080
breathing, sensing, growing, or fighting infection?

00:16:23.540 --> 00:16:25.180
Go ahead and take your guess and then Shelly

00:16:25.180 --> 00:16:26.840
will let us know the answer.

00:16:26.840 --> 00:16:28.460
And then we'll figure out which one you think is

00:16:28.460 --> 00:16:29.900
the least important.

00:16:35.660 --> 00:16:38.260
Alright, we've got over seventy-five percent of you

00:16:38.260 --> 00:16:40.160
that have voted. We'll just wait another couple

00:16:40.160 --> 00:16:43.000
of seconds here to allow more people

00:16:43.100 --> 00:16:45.100
participation opportunity.

00:16:46.640 --> 00:16:49.420
Alright, closing the poll and sharing the results.

00:16:49.420 --> 00:16:52.760
So, we have sixty-two percent of our participants

00:16:53.280 --> 00:16:56.580
saying breathing, with the next highest being

00:16:56.580 --> 00:16:59.060
twenty-one percent saying growing.

00:17:03.480 --> 00:17:05.260
So, go ahead. Do you want to do the

00:17:05.260 --> 00:17:06.340
second part question before we -

00:17:06.340 --> 00:17:07.920
- Yeah, we can just go ahead and yeah.

00:17:07.920 --> 00:17:10.280
- Okay, ooh this is gonna be tricky, right?

00:17:10.280 --> 00:17:12.940
So, sorry about the misspelling there but go ahead

00:17:12.940 --> 00:17:15.360
and let us know which processes you think

00:17:15.360 --> 00:17:16.720
is least important.

00:17:18.140 --> 00:17:20.260
Another chance to vote.

00:17:21.820 --> 00:17:25.400
The same processes are listed, so this is tricky

00:17:25.400 --> 00:17:27.440
because we didn't get the response to the first part

00:17:27.440 --> 00:17:28.560
of the question.

00:17:32.960 --> 00:17:35.620
Alright, just over seventy percent have voted.

00:17:43.140 --> 00:17:46.160
Sharing the results. So, now least important:

00:17:46.160 --> 00:17:49.120
sensing got forty-six percent and growing got

00:17:49.120 --> 00:17:52.980
thirty-seven percent. So, now we get to hear the

00:17:52.980 --> 00:17:55.280
response, the correct answers, Shelly.

00:17:56.080 --> 00:17:59.000
- Cool, yeah. So that's, it's really, I just want to say

00:17:59.440 --> 00:18:02.140
it's really interesting where the exercise we just did,

00:18:02.300 --> 00:18:05.780
like looking at that, because that's a lot of times

00:18:05.780 --> 00:18:09.620
like what we try to do as biologists is interpret

00:18:09.960 --> 00:18:11.980
what might be these most important processes.

00:18:11.980 --> 00:18:15.320
That's how we come up with a hypothesis of what to

00:18:15.480 --> 00:18:18.260
gear our research towards.

00:18:18.260 --> 00:18:20.900
So, kind of trying to figure out what are the vital

00:18:20.900 --> 00:18:24.260
processes and then look at those to see what

00:18:24.760 --> 00:18:27.160
might be affected.

00:18:27.160 --> 00:18:30.800
Like I was saying earlier, so these

00:18:30.800 --> 00:18:34.720
environmental stress like ocean acidification

00:18:35.080 --> 00:18:40.060
can cause a diversion of cellular energy to where

00:18:42.500 --> 00:18:45.180
the animal is gonna have to decide how much

00:18:47.060 --> 00:18:49.340
energy am I gonna put towards breathing?

00:18:49.340 --> 00:18:51.320
Maybe in ocean acidification, the animal has

00:18:51.640 --> 00:18:54.780
to put more energy into breathing and can't put as

00:18:54.900 --> 00:18:57.140
much energy into other things, like growing.

00:18:57.140 --> 00:19:00.860
So maybe the animal could end up being smaller, or

00:19:02.020 --> 00:19:03.520
maybe the animal might not be able to

00:19:03.520 --> 00:19:06.640
fight off the infection as well as it could before.

00:19:07.580 --> 00:19:10.500
Or maybe it has to sacrifice some of its sensing

00:19:10.520 --> 00:19:12.860
to be able to support the energy required to deal with

00:19:13.000 --> 00:19:15.040
the environmental stress.

00:19:17.580 --> 00:19:19.700
So, the questions were kind of more

00:19:19.700 --> 00:19:23.260
meant as an exercise and were kind of investigating

00:19:23.420 --> 00:19:26.080
the processes that are most important

00:19:26.080 --> 00:19:27.880
for the animal to be doing.

00:19:27.960 --> 00:19:32.260
Definitely, breathing is an important process, though.

00:19:32.340 --> 00:19:36.020
So, I would say that's correct for the first one.

00:19:37.240 --> 00:19:40.340
Then I'm just gonna talk a little bit about

00:19:40.560 --> 00:19:45.400
where crabs live and their natural habitat.

00:19:46.480 --> 00:19:48.280
They're commonly found from Alaska to

00:19:48.280 --> 00:19:53.120
southern California, and live in sea grass beds

00:19:55.180 --> 00:19:58.220
or the sandy floor and typically from the

00:19:58.220 --> 00:20:01.200
sea shore to three-hundred feet deep.

00:20:01.200 --> 00:20:04.660
And the Pacific Northwest coast is naturally more

00:20:04.660 --> 00:20:06.580
acidic than other coastal regions, Jenny kind of

00:20:06.580 --> 00:20:09.880
alluded to that, like where the west coast of the U.S.

00:20:09.880 --> 00:20:14.660
is particularly acidic compared to other regions.

00:20:16.440 --> 00:20:19.660
Or has a typically lower pH and that is due to this

00:20:19.660 --> 00:20:21.760
regional upwelling that happens along the

00:20:21.840 --> 00:20:23.600
west coast here.

00:20:24.880 --> 00:20:28.480
So, we expect that ocean acidification in this region

00:20:28.480 --> 00:20:30.640
will lead to more extreme conditions.

00:20:30.660 --> 00:20:33.620
So, where the pH is becoming lower globally,

00:20:33.620 --> 00:20:36.760
it's becoming even lower in the west coast region,

00:20:36.760 --> 00:20:39.400
which is where the dungeness crab live.

00:20:40.540 --> 00:20:44.240
And something to think about is, like humans,

00:20:44.340 --> 00:20:46.380
crabs are sensitive to different

00:20:46.380 --> 00:20:48.240
environmental conditions.

00:20:48.240 --> 00:20:52.640
And they have these thresholds like we do.

00:20:52.640 --> 00:20:55.260
If we get pushed beyond our physical limit,

00:20:55.260 --> 00:20:57.760
we can get stressed.

00:20:58.180 --> 00:21:01.720
So, for instance, if the temperature is too warm,

00:21:01.720 --> 00:21:06.840
they can develop hypothermia, or sorry, hyperthermia.

00:21:07.100 --> 00:21:12.440
And if the sea water pH becomes too low, they

00:21:12.560 --> 00:21:16.600
could develop acidosis, and also, if the sea water

00:21:16.700 --> 00:21:19.060
oxygen saturation becomes too low, they can

00:21:19.060 --> 00:21:21.380
develop hypoxia response.

00:21:22.180 --> 00:21:27.880
And just for reference, this is today's typical ocean

00:21:28.200 --> 00:21:32.260
chemistry, and the year 2100 projections.

00:21:33.460 --> 00:21:37.240
And so one thing that we're interested in is trying

00:21:37.240 --> 00:21:39.880
to understand what are the thresholds of these

00:21:39.880 --> 00:21:44.500
animals. Are these future ranges gonna push

00:21:44.500 --> 00:21:47.260
the animals past their thresholds or not?

00:21:47.260 --> 00:21:48.760
Are they gonna be able to adapt?

00:21:49.640 --> 00:21:51.420
So, those are some kinds of things we're

00:21:51.420 --> 00:21:54.280
hoping to address in the research that we do.

00:21:58.240 --> 00:22:00.200
This is just the dungeness crab

00:22:00.200 --> 00:22:04.480
life cycle here. So, they start off as these tiny eggs

00:22:05.740 --> 00:22:08.280
on the female, they carry them on the outside of

00:22:08.280 --> 00:22:10.660
their body for about three to six months.

00:22:10.660 --> 00:22:13.460
And then they hatch into this first larval stage

00:22:13.460 --> 00:22:18.020
called zoea, and during this stage, they actually have

00:22:18.840 --> 00:22:22.820
five different moltings that they go through, molts

00:22:22.820 --> 00:22:26.300
that they go through, which takes three to six months

00:22:27.140 --> 00:22:29.380
to complete all of these molts.

00:22:30.160 --> 00:22:34.160
And then they enter into this late larval stage called

00:22:35.700 --> 00:22:38.740
the megalopa stage or megalopal larvae stage.

00:22:39.200 --> 00:22:42.280
And so in both of these stages, these animals are

00:22:42.280 --> 00:22:45.180
swimming in the water column.

00:22:48.160 --> 00:22:51.660
And then after this late larval stage, they kind of

00:22:51.660 --> 00:22:55.280
settle out to the bottom and molt into these

00:22:56.240 --> 00:22:58.940
juvenile crabs. And they're juvenile crabs for about

00:22:58.940 --> 00:23:03.700
one to two years, after which they molt into adult crabs.

00:23:05.380 --> 00:23:08.960
And during the juvenile years, they molt more

00:23:09.220 --> 00:23:11.920
frequently than when they are adults.

00:23:11.920 --> 00:23:14.560
And when they're adults, they molt about once a year.

00:23:15.420 --> 00:23:20.280
And then once they're two years old, they can mate.

00:23:20.280 --> 00:23:22.640
They're sexually mature and can mate and start

00:23:22.640 --> 00:23:24.520
the cycle over again.

00:23:25.320 --> 00:23:28.460
So, I have another question: Which crab life stage

00:23:28.520 --> 00:23:31.000
do you think might be the most vulnerable

00:23:31.000 --> 00:23:32.680
to ocean acidification?

00:23:33.780 --> 00:23:35.060
- (Claire Fackler speaking) Alright, it's been launched

00:23:35.060 --> 00:23:36.720
on your screen, so

00:23:36.840 --> 00:23:38.880
we're gonna the answer for this one,

00:23:38.880 --> 00:23:41.680
I believe, after we do this poll.

00:23:42.880 --> 00:23:45.180
But you got the sense from that previous discussion

00:23:45.180 --> 00:23:48.080
with our two-part processes questions, right?

00:23:48.080 --> 00:23:50.540
The struggle of a scientist trying to figure out which

00:23:50.540 --> 00:23:53.380
processes might have the biggest influence, and

00:23:53.380 --> 00:23:54.820
which have the least.

00:23:55.460 --> 00:23:58.600
Alright, anyhow we are seventy percent, so there's

00:23:58.600 --> 00:24:03.640
still thirty percent of you that can choose to vote.

00:24:04.240 --> 00:24:05.200
So, please do.

00:24:06.240 --> 00:24:08.520
I'll go ahead and close the poll and share the results

00:24:08.520 --> 00:24:09.540
with everybody.

00:24:11.200 --> 00:24:13.020
We kind of have a mixed bag here, but almost

00:24:13.020 --> 00:24:16.620
fifty percent say the early larval stage, the zoe-ay,

00:24:16.820 --> 00:24:18.400
did I say that right?

00:24:18.400 --> 00:24:19.400
- Zoea.

00:24:19.400 --> 00:24:23.740
- Zoea. And then we have kind of an even mix

00:24:23.740 --> 00:24:26.440
between all categories but adult.

00:24:26.640 --> 00:24:29.760
So, no one, I guess two percent said adults, but

00:24:30.100 --> 00:24:32.200
seventeen to eighteen percent for the developing

00:24:32.200 --> 00:24:35.720
eggs and the late larval stage and juvenile.

00:24:37.620 --> 00:24:39.620
So, I'll let you interpret that.

00:24:41.400 --> 00:24:44.820
- Get to this one. So we so far have been thinking

00:24:44.820 --> 00:24:48.740
that the crabs are most likely

00:24:48.900 --> 00:24:52.780
vulnerable during this early larval stage because

00:24:52.780 --> 00:24:56.760
they're so small and there's a lot of pressure for

00:24:56.760 --> 00:25:00.360
them to get bigger because this is really,

00:25:00.940 --> 00:25:03.100
in their zoea stage and megalopa stage

00:25:03.100 --> 00:25:05.940
when they are vulnerable to predators

00:25:06.580 --> 00:25:08.580
more so than in an adult stage.

00:25:09.060 --> 00:25:11.940
So, there's this pressure on the animal to grow, so

00:25:11.940 --> 00:25:15.140
if the animal has all this

00:25:15.260 --> 00:25:17.560
pressure to grow, are they gonna be able to deal

00:25:17.560 --> 00:25:19.360
with the environmental stress, as well?

00:25:20.140 --> 00:25:22.680
Where, once they're an adult,

00:25:22.820 --> 00:25:25.600
and they have a hard shell

00:25:25.600 --> 00:25:27.540
they don't have to put as

00:25:27.540 --> 00:25:30.880
much energy into their defense as they do at

00:25:30.880 --> 00:25:34.560
this (zoea) stage, with their defense being like size.

00:25:35.780 --> 00:25:38.040
But we are still trying to understand

00:25:38.040 --> 00:25:40.400
all these different life stages and where ocean

00:25:40.400 --> 00:25:45.240
acidification might be affecting the animal most.

00:25:48.320 --> 00:25:51.280
So, there's two different ways that we can

00:25:51.280 --> 00:25:54.680
study how the crabs might be dealing with

00:25:55.020 --> 00:25:57.820
ocean chemistry change, and that's by doing field

00:25:57.820 --> 00:26:00.420
experiments and laboratory experiments.

00:26:00.420 --> 00:26:03.640
An example of a field experiment can be

00:26:04.420 --> 00:26:07.540
comparing two different populations of crabs

00:26:07.600 --> 00:26:11.440
maybe from regions that vary in pH.

00:26:12.800 --> 00:26:14.720
For instance, in Hood Canal, Washington there's

00:26:14.720 --> 00:26:18.540
a lower pH there than, for instance on the coast

00:26:18.540 --> 00:26:20.080
of Washington.

00:26:20.080 --> 00:26:22.120
So, we might compare two different populations

00:26:22.120 --> 00:26:26.400
from there and see what their response might be to

00:26:26.400 --> 00:26:29.140
infection, say, just as an example.

00:26:30.820 --> 00:26:33.460
One thing that's complicate about, oh and then there's

00:26:33.460 --> 00:26:38.000
these, we also have these nice buoys that record

00:26:38.160 --> 00:26:42.900
ocean chemistry data continuously so that we're

00:26:42.900 --> 00:26:46.280
able to make associations with the population and

00:26:46.280 --> 00:26:48.120
the ocean chemistry there.

00:26:48.380 --> 00:26:50.860
But one tricky thing about these field experiments

00:26:50.860 --> 00:26:54.780
is that it's not the conditions, there can be other things

00:26:54.780 --> 00:26:58.360
about the regions that influence the results

00:26:58.360 --> 00:27:01.600
that we see. So that's where laboratory experiments

00:27:01.600 --> 00:27:05.040
are really nice because we can finally control the

00:27:05.300 --> 00:27:08.600
pH, temperature, and dissolved gasses and not worry

00:27:08.600 --> 00:27:11.520
about other things that are happening in the area,

00:27:11.520 --> 00:27:14.980
such as run-off or

00:27:15.560 --> 00:27:18.620
environmental pollution and that kind of stuff.

00:27:20.020 --> 00:27:22.640
So, that's why we, these laboratory experiments

00:27:22.640 --> 00:27:25.680
kind of make it simpler to answer some

00:27:25.840 --> 00:27:28.260
questions that we have.

00:27:29.240 --> 00:27:32.100
So, one way that NOAA at the Northwest Fisheries

00:27:32.100 --> 00:27:37.140
Science Center is assessing carbon dioxide

00:27:37.300 --> 00:27:40.500
sensitivity in dungeness crab is by collecting

00:27:40.540 --> 00:27:45.320
different crabs. So, some work that I was involved in

00:27:45.320 --> 00:27:53.180
last year was looking at these, so

00:27:54.000 --> 00:27:58.960
they collect female crabs carrying fertilized eggs

00:27:58.960 --> 00:28:02.160
and then hatch the eggs in the lab and then rear

00:28:02.160 --> 00:28:06.480
these crabs, raise these crabs in different

00:28:07.280 --> 00:28:10.160
ocean acidification conditions.

00:28:10.160 --> 00:28:14.400
And then we've also collected crabs at this

00:28:14.400 --> 00:28:19.880
megalopa stage and then, so when I say rear the

00:28:19.880 --> 00:28:24.500
larvae in different conditions, they do this at the

00:28:24.500 --> 00:28:27.600
center by using these mobile ocean acidification

00:28:27.600 --> 00:28:31.140
treatment systems. So, where each one of these

00:28:31.140 --> 00:28:33.100
systems, so each one of these computers, controls

00:28:33.100 --> 00:28:36.060
a different system and there is flowing filtered sea

00:28:36.060 --> 00:28:39.840
water going through it, where you can mix different

00:28:39.840 --> 00:28:41.700
amounts of carbon dioxide and sea water

00:28:42.700 --> 00:28:45.200
and see how that is affecting the development

00:28:45.200 --> 00:28:46.700
of the crabs.

00:28:46.760 --> 00:28:49.540
So, now I'm gonna show this video kind of like

00:28:49.540 --> 00:28:52.660
the process of what that looks like.

00:28:53.940 --> 00:28:58.280
So, in this first clip, NOAA scientists are collecting

00:28:58.280 --> 00:29:00.660
the late larval stage crabs and

00:29:00.660 --> 00:29:02.180
now they're pulling them back

00:29:02.180 --> 00:29:06.100
up onto the NOAA pier in the bucket and that's all

00:29:06.100 --> 00:29:08.420
these larvae that were collected.

00:29:09.880 --> 00:29:14.040
And then each larvae is gonna go into their own jars

00:29:15.460 --> 00:29:18.580
and put onto a treatment, and this allows us to

00:29:18.580 --> 00:29:21.680
monitor the development of an individual crab.

00:29:22.460 --> 00:29:24.980
So, in the field that would be extremely hard to do,

00:29:24.980 --> 00:29:26.260
if not impossible.

00:29:27.180 --> 00:29:29.660
And then we check the development over time

00:29:29.660 --> 00:29:32.040
to see and record when they've molted.

00:29:32.040 --> 00:29:33.840
Oops, sorry guys.

00:29:33.840 --> 00:29:34.560
 

00:29:34.560 --> 00:29:37.200
Let me go right back to there.

00:29:37.820 --> 00:29:40.680
So, you can see this crab has molted into a

00:29:40.960 --> 00:29:42.860
juvenile crab and then we write down

00:29:42.860 --> 00:29:45.580
the dates that that molting occurred on.

00:29:50.960 --> 00:29:54.720
So, in these experiments we can simulate the

00:29:54.760 --> 00:30:00.000
future ocean pH and the current ocean pH and

00:30:01.080 --> 00:30:04.360
measure how much energy the larvae are producing

00:30:05.080 --> 00:30:07.760
throughout the treatment, or the length of time that

00:30:07.760 --> 00:30:11.600
they're on these treatments by measuring their

00:30:11.600 --> 00:30:16.520
metabolic rate. We can count the number of crabs

00:30:16.760 --> 00:30:20.040
that survive in each treatment, measure the size.

00:30:21.160 --> 00:30:22.800
We can also measure the activity of different

00:30:22.800 --> 00:30:24.900
biochemical pathways happening inside

00:30:24.960 --> 00:30:27.040
the crab by doing this cool thing called

00:30:27.040 --> 00:30:30.480
metabolomics. And we can also look at the crab's DNA

00:30:30.640 --> 00:30:33.920
and compare, look and see if there's changes,

00:30:34.200 --> 00:30:37.440
if the treatment may have induced different

00:30:37.440 --> 00:30:41.000
epigenetic changes or genetic changes, which I'm

00:30:41.000 --> 00:30:44.000
gonna talk about in a couple slides.

00:30:44.200 --> 00:30:45.980
With the overall goal of being able to

00:30:45.980 --> 00:30:47.220
make more accurate

00:30:47.220 --> 00:30:50.400
predictions of whether the animal will be able to

00:30:50.540 --> 00:30:54.740
acclimate. So, this is some data that was published

00:30:54.740 --> 00:30:58.920
two years ago, it's a NOAA study, that showed low pH

00:30:59.060 --> 00:31:01.460
decreases dungeness crab larval

00:31:01.460 --> 00:31:03.460
survival and development.

00:31:04.160 --> 00:31:09.500
So, in the lower pH sea water, less larvae survived

00:31:09.600 --> 00:31:13.340
compared to the ambient pH sea water.

00:31:14.220 --> 00:31:19.100
Another measurement that was done was measuring

00:31:19.100 --> 00:31:22.720
the size of the zoea and this is just showing

00:31:22.720 --> 00:31:25.300
how you can do that.

00:31:26.460 --> 00:31:31.580
And then this is a project I worked on this past year

00:31:32.100 --> 00:31:35.460
that we just submitted for publication, and this was

00:31:35.460 --> 00:31:37.580
doing that cool technique called metabolomics,

00:31:37.580 --> 00:31:39.960
where we're able to measure all these different

00:31:39.960 --> 00:31:41.920
metabolites in the crab.

00:31:41.920 --> 00:31:44.960
So we had a couple different treatments and

00:31:45.200 --> 00:31:48.820
compared to our ambient treatment or the

00:31:49.260 --> 00:31:53.040
normal ocean conditions, we saw that in response

00:31:53.040 --> 00:31:58.280
to low pH, the animals showed citric acid cycle,

00:31:58.300 --> 00:32:02.100
increased activity. In response to low oxygen, they

00:32:02.100 --> 00:32:05.580
showed a hypoxia stress response that was common

00:32:05.580 --> 00:32:08.780
in other animals that were exposed to low oxygen.

00:32:09.820 --> 00:32:14.060
And then in a combined low oxygen and pH stress,

00:32:14.240 --> 00:32:20.020
we saw that low oxygen, the response to low oxygen

00:32:20.180 --> 00:32:23.660
dominated the pH response.

00:32:27.680 --> 00:32:29.740
I guess I want to ask this, this is the last

00:32:29.740 --> 00:32:31.160
polling question.

00:32:31.160 --> 00:32:35.720
Now, after seeing that data, what does everyone think?

00:32:35.720 --> 00:32:39.400
Are dungeness crabs affected by ocean acidification?

00:32:40.960 --> 00:32:41.860
- (Claire Fackler speaking) Alright, if you haven't

00:32:41.860 --> 00:32:43.840
seen it already, the quick poll is up there,

00:32:43.880 --> 00:32:48.300
so go ahead and vote on your prediction.

00:32:51.300 --> 00:32:52.780
We'll give folks just another couple

00:32:52.780 --> 00:32:56.540
seconds here to get us into the majority.

00:33:00.680 --> 00:33:03.180
Alright, just about eighty percent have voted, so

00:33:03.180 --> 00:33:05.880
I will close the poll and here are the results.

00:33:06.060 --> 00:33:09.320
So, ninety-three percent are saying absolutely yes,

00:33:09.320 --> 00:33:12.860
and there's seven percent saying we don't know yet.

00:33:13.300 --> 00:33:18.280
- Okay, so based on our data, those are, the

00:33:19.060 --> 00:33:23.980
experiments that I showed are laboratory results,

00:33:24.220 --> 00:33:28.100
and CO2 exposure experiments in filtered sea water.

00:33:28.100 --> 00:33:31.540
So, it's ocean acidification simulated like data.

00:33:33.340 --> 00:33:38.160
So, we don't yet know about ocean acidification, like

00:33:38.280 --> 00:33:41.580
in the actual ocean how the dungeness crab are

00:33:41.580 --> 00:33:43.200
going to respond.

00:33:43.200 --> 00:33:47.900
Based on our data, it seems like they, like in the, here

00:33:47.920 --> 00:33:51.460
I'm just gonna go back really quick just to show.

00:33:51.460 --> 00:33:55.640
So, based on this data, it seems like it might have

00:33:55.640 --> 00:33:58.640
a negative impact on them, but we don't actually

00:33:58.640 --> 00:34:01.860
know what would happen in the real ocean.

00:34:04.080 --> 00:34:07.100
So, we just have to bear that in mind in these

00:34:07.100 --> 00:34:09.580
experiments, they help kind of give us an idea of

00:34:09.580 --> 00:34:12.440
what might happen, but we still need to do some more

00:34:12.440 --> 00:34:15.960
experiments to find out, you know, get closer

00:34:16.080 --> 00:34:17.620
to the truth.

00:34:18.140 --> 00:34:21.680
So, there is ongoing research at NOAA so we can

00:34:21.680 --> 00:34:26.260
try to figure this out, and one thing is this

00:34:27.660 --> 00:34:29.640
megalopa shell quality study.

00:34:29.640 --> 00:34:32.920
So, this is, there was this cruise that went

00:34:32.920 --> 00:34:37.720
up the west coast, and

00:34:38.300 --> 00:34:40.080
these late larval stage crabs

00:34:40.080 --> 00:34:41.360
were collected in these different

00:34:41.360 --> 00:34:46.760
sites, and so what they're working on is trying to

00:34:46.760 --> 00:34:50.600
see if their shells, if the quality of their shells is

00:34:50.600 --> 00:34:53.320
different depending on the region that they were

00:34:53.320 --> 00:34:58.700
collected in, and see if that is associated with

00:34:58.700 --> 00:35:01.180
regional pH.

00:35:01.360 --> 00:35:04.500
And then, the center is also working on this

00:35:04.500 --> 00:35:06.260
multi-generational study.

00:35:06.260 --> 00:35:08.720
So, the other studies that I showed you, one was

00:35:08.720 --> 00:35:12.760
from, so the metabolomics work was in juvenile crab

00:35:13.480 --> 00:35:18.560
and the first study was in early larval stage crabs.

00:35:18.560 --> 00:35:22.120
So, this current study that's going on is looking

00:35:22.120 --> 00:35:27.460
across, the goal is to look across generations.

00:35:28.000 --> 00:35:32.220
So, where different animals can be collected, so

00:35:32.220 --> 00:35:35.480
different adult crabs can be, adult females can be

00:35:35.480 --> 00:35:42.180
collected from different regions and raise their larvae

00:35:42.640 --> 00:35:48.800
in low pH conditions or normal pH conditions over

00:35:48.900 --> 00:35:51.400
the course of their life.

00:35:51.540 --> 00:35:55.500
So, from larvae stage to juvenile and then to adult

00:35:56.100 --> 00:35:58.880
and see how the animals do over the course of

00:35:58.880 --> 00:36:00.760
their different life stages when they're in these

00:36:00.760 --> 00:36:02.360
different conditions.

00:36:02.360 --> 00:36:06.740
And then see if their region of origin is connected

00:36:06.740 --> 00:36:09.540
with how they end up doing in response to the

00:36:09.540 --> 00:36:12.780
different conditions they're put in.

00:36:15.420 --> 00:36:19.720
I feel like I kind of went fast, so I expect questions

00:36:19.720 --> 00:36:24.140
at the end, but that was kind of the end of the

00:36:24.140 --> 00:36:27.440
NOAA research part and now I'm just gonna do this

00:36:27.440 --> 00:36:30.860
overview of the national marine sanctuaries

00:36:30.860 --> 00:36:33.660
dungeness crab ocean acidification toolkit.

00:36:39.200 --> 00:36:41.060
So, I just wanted to kind of talk about, so these are

00:36:41.060 --> 00:36:43.200
the different pieces in the toolkit.

00:36:43.200 --> 00:36:48.680
If you go to this website, you can find a fact sheet,

00:36:48.680 --> 00:36:53.980
which is a double-page sheet with a lot of the

00:36:54.080 --> 00:36:57.040
information that was in this presentation.

00:36:57.240 --> 00:37:01.400
And you might want to use that for a handout at

00:37:01.400 --> 00:37:04.360
an event or something where you're just trying to

00:37:04.360 --> 00:37:07.840
convey or get this information out to people.

00:37:08.280 --> 00:37:11.980
There's also this powerpoint slideshow that has

00:37:11.980 --> 00:37:13.380
a script with it.

00:37:13.380 --> 00:37:17.280
A lot of the slides that I show today are part of this

00:37:17.280 --> 00:37:19.940
slideshow, I think ninety percent of the slides that

00:37:19.940 --> 00:37:25.280
I showed are from here, for classroom or whatever

00:37:25.280 --> 00:37:28.700
purposes, and if you wanted to just show part of it,

00:37:28.700 --> 00:37:31.460
because there's more slides in this slideshow that

00:37:31.460 --> 00:37:36.360
kind of talk about more about the cultural piece, the

00:37:36.360 --> 00:37:40.100
economic piece, and if you wanted to take different

00:37:40.100 --> 00:37:43.060
parts of the slideshow out and present it, you can

00:37:43.200 --> 00:37:44.520
do that.

00:37:44.640 --> 00:37:51.240
And then there's also this infographic that can be used,

00:37:51.240 --> 00:37:55.020
there are public domain photos that you can add

00:37:55.020 --> 00:37:58.540
to your presentation or create something else with.

00:37:59.120 --> 00:38:04.520
There is this eight-minute long video B-roll which

00:38:04.520 --> 00:38:08.280
shows different clips, and there's explanations

00:38:08.680 --> 00:38:13.540
for each clip, so if you wanted to cut, so the video

00:38:13.560 --> 00:38:17.200
that I showed today was from here, it was sped up

00:38:17.760 --> 00:38:21.200
but I just took little clips from this B roll, so you

00:38:21.200 --> 00:38:23.860
could something like that if you wanted to, or if you

00:38:23.900 --> 00:38:26.840
just wanted to show your classroom or whoever

00:38:26.840 --> 00:38:29.580
some information about the dungeness crab,

00:38:29.580 --> 00:38:32.920
you can go here, and then there's also this reference

00:38:32.920 --> 00:38:37.440
list, which includes all the sources that are in the

00:38:37.640 --> 00:38:40.240
presentation fact sheet, etc.

00:38:40.500 --> 00:38:43.360
So, if you want to go back to the studies that are

00:38:43.360 --> 00:38:45.660
referenced or where does this information come

00:38:45.660 --> 00:38:48.480
from about how much carbon dioxide is in the air

00:38:48.480 --> 00:38:50.980
and that kind of stuff, you can just check on this

00:38:51.280 --> 00:38:54.440
reference list and go back to the original sources.

00:38:55.160 --> 00:38:58.440
And then there's also this really nice resource list, if

00:38:58.440 --> 00:39:02.520
you're interested in more what's going on at NOAA,

00:39:02.520 --> 00:39:04.980
what's going on with the NOAA ocean acidification

00:39:04.980 --> 00:39:07.180
program, what are your national marine sanctuaries

00:39:07.180 --> 00:39:10.340
doing, what are ways that you can get involved,

00:39:10.340 --> 00:39:12.460
what are events that are happening around ocean

00:39:12.460 --> 00:39:15.320
acidification, all that kind of information you can

00:39:15.480 --> 00:39:18.460
just check out, definitely check out the resource list.

00:39:24.380 --> 00:39:26.960
And I think that's it.

00:39:27.640 --> 00:39:31.280
- (Claire Fackler speaking) Excellent, well thank you, Doctor Trigg, for your

00:39:31.280 --> 00:39:32.800
presentation.

00:39:32.800 --> 00:39:34.960
I'll pop up my web camera and join you again, and

00:39:34.960 --> 00:39:36.660
maybe Jenny, you can, too, because perhaps between

00:39:36.660 --> 00:39:37.980
the three of us, we can answer

00:39:37.980 --> 00:39:39.380
some of these questions.

00:39:39.380 --> 00:39:41.000
So, if you haven't already, go ahead and type your

00:39:41.000 --> 00:39:42.600
question into the question box.

00:39:42.600 --> 00:39:45.140
If you're feeling brave, look in your control panel,

00:39:45.340 --> 00:39:46.180
if there's a little hand

00:39:46.180 --> 00:39:49.140
there that you can raise, which means that I will

00:39:49.140 --> 00:39:52.380
un-mute you and you can actually have audio and

00:39:52.380 --> 00:39:55.160
ask your question, but I know on these webinars and

00:39:55.160 --> 00:39:57.780
past experience not too many people raise their hand.

00:39:57.780 --> 00:39:59.120
They'd much rather type it.

00:39:59.120 --> 00:40:02.060
So, our first question that has come in is, "What

00:40:02.060 --> 00:40:04.600
about the issues that oyster farms on the west

00:40:04.600 --> 00:40:06.600
coast are presently having?"

00:40:07.020 --> 00:40:10.360
So, can you shed some light onto that, Shelly?

00:40:10.360 --> 00:40:15.320
- Okay, so what I know about the oyster farms, so

00:40:15.740 --> 00:40:19.020
oyster shells, I thought about including something

00:40:19.020 --> 00:40:23.260
about this, oyster shells are calcium carbonate.

00:40:23.740 --> 00:40:25.940
A lot of bivalve shells are made

00:40:25.980 --> 00:40:27.160
out of calcium carbonate,

00:40:27.160 --> 00:40:29.320
and that's different than what crab, dungeness crab

00:40:29.320 --> 00:40:32.520
shells are made out of chitin and it's a stronger

00:40:32.780 --> 00:40:38.980
material, so the calcium carbonate shells are a little

00:40:38.980 --> 00:40:44.100
more affected by the low pH as far as we see in the

00:40:44.180 --> 00:40:51.340
lab, and I know in some of the hatcheries and stuff

00:40:51.980 --> 00:40:56.020
that had a problem when there was a natural

00:40:56.020 --> 00:41:00.880
low pH event happen, and had very low

00:41:01.380 --> 00:41:05.860
survivorship, I think there were some

00:41:05.860 --> 00:41:09.340
cases where they were able to buffer the water

00:41:09.340 --> 00:41:11.180
once they were able to figure out what that was, they

00:41:11.180 --> 00:41:14.320
were able to buffer their water that was coming in

00:41:14.600 --> 00:41:18.000
and saw increased survival to that.

00:41:18.000 --> 00:41:20.480
That's just one example, though, I'm not sure about

00:41:20.480 --> 00:41:22.360
all the problems.

00:41:23.540 --> 00:41:24.800
- Excellent. Well, yes,

00:41:24.800 --> 00:41:25.840
and your work was on the

00:41:25.840 --> 00:41:27.200
dungeness crab but you do have some insight

00:41:27.200 --> 00:41:28.580
to that, thank you.

00:41:28.940 --> 00:41:32.460
So, John Winneck, you have raised your hand, lucky

00:41:32.460 --> 00:41:33.900
you, I'm gonna un-mute you,

00:41:33.900 --> 00:41:35.500
let's see if this is gonna work.

00:41:37.240 --> 00:41:39.800
Alright, John, what's your question?

00:41:41.420 --> 00:41:43.180
Are you there?

00:41:43.640 --> 00:41:46.580
Okay, we're going to mute you, John.

00:41:46.580 --> 00:41:48.540
We'll try again if you keep your hand raised.

00:41:48.540 --> 00:41:51.040
Is there anyone else with a raised hand?

00:41:51.040 --> 00:41:52.720
If not, I'll go to one of our additional

00:41:52.720 --> 00:41:54.340
typed questions here.

00:41:54.700 --> 00:41:58.100
We have, this is one that maybe even Jenny and I

00:41:58.100 --> 00:42:01.200
can pipe in. If you were to explain ocean

00:42:01.200 --> 00:42:04.280
acidification to elementary school kids, is there

00:42:04.280 --> 00:42:07.760
one talking point or to-do or even visual from the

00:42:07.760 --> 00:42:11.200
toolkit or elsewhere that this teacher or teachers

00:42:11.280 --> 00:42:13.360
of second-graders should use?

00:42:13.980 --> 00:42:15.940
Not second-graders, elementary school students,

00:42:15.940 --> 00:42:17.940
so K through six.

00:42:18.720 --> 00:42:20.740
We'll let you start, Shelly, and then Jenny, if you have

00:42:20.740 --> 00:42:22.920
anything you'd like to add.

00:42:25.980 --> 00:42:29.020
- One piece of the toolkit that might be better at

00:42:29.080 --> 00:42:33.240
reaching young, young students.

00:42:34.140 --> 00:42:41.760
Let's see, I guess I think the visuals are really

00:42:41.760 --> 00:42:47.820
helpful, so maybe part of the video.

00:42:49.160 --> 00:42:54.560
Ocean acidification is a complex concept, so it is

00:42:54.560 --> 00:42:58.800
tricky, but I think sometimes seeing the live footage

00:42:58.800 --> 00:43:02.380
of what's happening and just thinking about it

00:43:02.380 --> 00:43:03.740
that way might help.

00:43:05.100 --> 00:43:07.760
- Yeah, and I think I would even add maybe just that

00:43:07.760 --> 00:43:12.640
life cycle visual is really helpful to discuss the life

00:43:12.640 --> 00:43:15.400
cycle, and then of course the chemistry of ocean

00:43:15.500 --> 00:43:18.580
acidification is pretty complex and for some of the

00:43:18.580 --> 00:43:21.060
younger elementary school students might be hard

00:43:21.060 --> 00:43:21.700
to grasp.

00:43:24.060 --> 00:43:30.340
- Maybe, I'm just gonna scroll back real quick, I know

00:43:30.340 --> 00:43:37.840
this is a complex figure, but it's kind of like, I tried

00:43:38.060 --> 00:43:41.720
to put it in perspective kind of like, when humans

00:43:41.720 --> 00:43:45.600
get too hot, we can not feel so good or if you have

00:43:46.500 --> 00:43:49.400
limited oxygen you don't feel so good, so I try to

00:43:49.560 --> 00:43:52.600
put it like that and I don't know, that might help.

00:43:54.420 --> 00:43:57.440
- And Jenny, you have experience in working with

00:43:57.440 --> 00:44:00.600
probably higher than elementary school teachers

00:44:00.600 --> 00:44:02.720
and students on O.A., but do you have any insight

00:44:02.720 --> 00:44:04.080
you'd like to share?

00:44:05.020 --> 00:44:08.680
- Yeah, I think ocean acidification, just like climate,

00:44:08.680 --> 00:44:12.760
is a really challenging topic, even for us adults to

00:44:12.760 --> 00:44:15.920
take it on in terms of really internalizing what the

00:44:15.920 --> 00:44:16.860
effects are to our lives.

00:44:18.140 --> 00:44:22.740
And in general, with younger students, I try not to

00:44:22.740 --> 00:44:26.300
add any traumatic information for anybody below

00:44:26.300 --> 00:44:30.060
fourth grade, and that is consistent with a lot of the

00:44:30.060 --> 00:44:33.660
early education literature that we really don't want

00:44:33.740 --> 00:44:37.960
to make it a traumatic event for younger kids.

00:44:37.960 --> 00:44:41.020
We want to them to fall in love with the mystery and

00:44:41.140 --> 00:44:43.880
the excitement and the beauty of the ocean at those

00:44:43.880 --> 00:44:46.380
younger ages.

00:44:46.380 --> 00:44:49.280
That said, I think it's safe in terms of the younger

00:44:49.280 --> 00:44:52.840
ages to talk about how the animals in the ocean

00:44:53.260 --> 00:44:55.660
need the conditions to be a certain way in order to

00:44:55.660 --> 00:44:59.100
live, they are in salt water and if it gets too hot for

00:44:59.100 --> 00:45:01.960
them, they have nowhere to go.

00:45:02.280 --> 00:45:04.660
So, I think just in terms of the younger kids, just

00:45:04.660 --> 00:45:06.640
talking about healthy environment, and the video

00:45:06.880 --> 00:45:10.740
footage is good to show what habitat crabs need

00:45:10.740 --> 00:45:12.740
and what it looks like to them.

00:45:14.740 --> 00:45:16.300
The simplest I've talked about with ocean

00:45:16.300 --> 00:45:19.740
acidification is just how the carbon dioxide in the

00:45:20.280 --> 00:45:22.600
atmosphere is being absorbed by the ocean, the

00:45:22.600 --> 00:45:25.700
ocean is the dominant habitat on our planet, and it

00:45:26.300 --> 00:45:32.280
acts like a sponge and absorbs this excess air into

00:45:32.500 --> 00:45:35.640
the ocean, and it's changing the chemistry of the

00:45:35.640 --> 00:45:38.580
ocean, making it harder for some organisms or

00:45:38.580 --> 00:45:43.280
animals to live, and just keeping it simple as that.

00:45:43.280 --> 00:45:45.920
And so we really need to conserve energy and put

00:45:45.920 --> 00:45:47.820
less of that carbon dioxide out there.

00:45:47.960 --> 00:45:49.920
And that's about as far as I've gotten with

00:45:49.920 --> 00:45:53.480
younger students, my son being a test case at

00:45:53.500 --> 00:45:56.860
seven years old, I try not to overdo it there.

00:45:56.920 --> 00:45:59.520
But I think it's a really good question, and I'm glad

00:45:59.520 --> 00:46:03.060
you asked it, and I just think keeping in mind not to

00:46:03.120 --> 00:46:06.200
scare younger kids but to get them excited about

00:46:06.200 --> 00:46:08.920
ecology and biodiversity is really the most important

00:46:08.920 --> 00:46:10.260
thing to do.

00:46:10.860 --> 00:46:14.160
- Excellent, yeah that definitely caught us on our toes.

00:46:14.160 --> 00:46:16.300
So, I'm gonna try the audio again because we have

00:46:16.300 --> 00:46:19.060
Paul McElheny, who has expressed interest in

00:46:19.060 --> 00:46:21.980
speaking, so you're gonna have to un-mute yourself,

00:46:21.980 --> 00:46:24.780
Paul, I've un-muted you, but now you're in control

00:46:24.780 --> 00:46:28.300
on your end, let's see, ooh I might strike out twice

00:46:28.300 --> 00:46:33.920
here; so in your control panel, Paul, you now have

00:46:33.920 --> 00:46:39.020
the capability of un-muting yourself.

00:46:40.680 --> 00:46:41.640
You're muted again, sorry.

00:46:41.640 --> 00:46:43.180
Okay, back to, we have tons of questions

00:46:43.180 --> 00:46:45.500
in the question box, so I'll stick with those for now.

00:46:46.220 --> 00:46:48.340
So, question for Shelly: What does an increase in

00:46:48.340 --> 00:46:52.100
citric acid cycle activity mean for a crab?

00:46:53.180 --> 00:47:01.240
- So, that means, in that low pH group, compared to

00:47:01.640 --> 00:47:06.740
the normal conditions, they were increasing kind of

00:47:06.740 --> 00:47:09.860
their energy production, so citric acid cycle is how

00:47:09.860 --> 00:47:15.280
the animal is making an energy molecule, which

00:47:15.280 --> 00:47:22.900
fuels a lot of biochemical reactions, and so it just

00:47:23.080 --> 00:47:27.860
means that they are trying to produce more fuel

00:47:27.860 --> 00:47:32.740
to support other things that are having to happen

00:47:32.740 --> 00:47:35.300
in the animal that are not happening in

00:47:36.160 --> 00:47:38.040
the normal conditions.

00:47:38.040 --> 00:47:40.840
I don't know if that helps.

00:47:40.840 --> 00:47:44.840
- Alright, well let's talk about, so apart from

00:47:44.840 --> 00:47:46.700
carbon emissions, what else can be eliminated

00:47:46.700 --> 00:47:49.760
to help reduce ocean acidification, so a more

00:47:49.880 --> 00:47:52.280
broad question here.

00:47:52.280 --> 00:47:54.480
- So, there's some different things, I'm just gonna

00:47:54.480 --> 00:47:58.920
talk about one example that's being pretty

00:47:58.920 --> 00:48:03.040
investigated right now, which is planting of sea

00:48:03.040 --> 00:48:07.540
grass in different regions.

00:48:08.240 --> 00:48:13.720
So, sea grass used to be more prevalent than it is

00:48:13.720 --> 00:48:17.760
in certain areas, and people are thinking if we put

00:48:18.600 --> 00:48:23.480
sea grass back, sea grass is a plant, so it will help

00:48:23.480 --> 00:48:33.060
absorb carbon and it will give off oxygen, so that's

00:48:33.480 --> 00:48:36.500
something that might help the situation that people

00:48:36.500 --> 00:48:43.640
are experimenting with trial out-plantings of it.

00:48:43.840 --> 00:48:46.480
So, we'll kind of see how that works; it's still

00:48:46.480 --> 00:48:48.640
definitely in early test phase.

00:48:50.060 --> 00:48:52.120
Something like that might work, but there's other

00:48:52.120 --> 00:48:53.900
things like that.

00:48:53.900 --> 00:48:56.520
- Alright, here's a couple questions directly related

00:48:56.520 --> 00:48:58.500
to your research: How did you manage to collect

00:48:58.500 --> 00:49:01.460
so many crab larvae from the ocean?

00:49:02.360 --> 00:49:05.420
And then, before you get there, a follow-up question

00:49:05.420 --> 00:49:07.840
is: When you finish raising the crabs in

00:49:07.840 --> 00:49:10.420
your lab study, do you release them

00:49:10.420 --> 00:49:12.800
back into the ocean?

00:49:13.100 --> 00:49:18.720
- So, the crab larvae, we have special traps to

00:49:19.180 --> 00:49:21.580
collect those, and we also have special permits

00:49:21.580 --> 00:49:28.460
that allow us to collect those, and that is just like

00:49:28.460 --> 00:49:30.000
a hit-or-miss thing.

00:49:30.000 --> 00:49:33.040
Sometimes there are no larvae for a couple weeks,

00:49:33.040 --> 00:49:38.500
and then there's this wave of lots of them, and they

00:49:38.500 --> 00:49:42.000
are attracted to light, so our traps have these light

00:49:42.000 --> 00:49:45.100
sources in them, and they just go into the trap and

00:49:45.100 --> 00:49:48.540
then can't get out. So for the larvae, for the animals,

00:49:48.540 --> 00:49:51.180
I guess this goes for the adults, too, when we take

00:49:51.180 --> 00:49:54.980
them into the lab and we're rearing them or raising

00:49:54.980 --> 00:50:00.040
them in our laboratory conditions, we don't release

00:50:00.040 --> 00:50:03.960
them back out to the wild in case anything might

00:50:03.960 --> 00:50:06.060
have happened to them in the lab.

00:50:06.060 --> 00:50:11.740
We would not want to risk if they for some reason,

00:50:11.740 --> 00:50:14.360
if they caught some infection from being in the lab,

00:50:14.360 --> 00:50:16.100
we would not want to release that back out

00:50:16.100 --> 00:50:18.860
to the wild, so we just try to, animals that we bring

00:50:18.860 --> 00:50:21.800
in we just use in our...

00:50:23.900 --> 00:50:29.000
- In the name of science.

00:50:30.100 --> 00:50:33.280
Are you collecting or receiving any data from the crab

00:50:33.280 --> 00:50:37.200
fisheries on changes in catch size or abundance?

00:50:37.240 --> 00:50:41.120
- Yeah, so there's people at the center who are doing

00:50:41.120 --> 00:50:48.140
analysis on that data that work with the

00:50:48.140 --> 00:50:54.620
Washington fish and wildlife data, or there's fish

00:50:54.620 --> 00:50:57.240
and wildlife data for the whole west coast that

00:50:57.240 --> 00:51:02.260
some people are doing analysis on to try to see if

00:51:02.260 --> 00:51:06.660
there are fluctuations in

00:51:06.660 --> 00:51:10.480
population size or estimate that.

00:51:10.960 --> 00:51:13.140
- And then have you seen population effects yet,

00:51:13.140 --> 00:51:16.700
like in recent, this is Ellen Hale saying in recent

00:51:16.700 --> 00:51:20.600
crab tissue sampling at a super fund site in

00:51:20.760 --> 00:51:24.000
Puget Sound they had a low number per effort

00:51:24.000 --> 00:51:26.060
of dungeness crabs, so have you seen any

00:51:26.060 --> 00:51:28.220
populations effects yourself?

00:51:29.980 --> 00:51:36.740
- Not that I am aware of, I know they've had to

00:51:36.740 --> 00:51:43.140
postpone season opening and that kind of stuff,

00:51:43.440 --> 00:51:47.260
I can't remember if that was last year, but sometimes

00:51:47.260 --> 00:51:50.180
that's happened, where it's like the animals, aren't

00:51:50.180 --> 00:51:51.840
it doesn't seem like they're big enough yet or their

00:51:51.840 --> 00:51:55.500
shells are too soft or too close, kind of like they

00:51:55.500 --> 00:51:59.080
molted a little later than expected.

00:51:59.840 --> 00:52:07.940
But we haven't noticed an overall population change,

00:52:09.440 --> 00:52:13.800
at least as far as I know what's been looked at.

00:52:17.320 --> 00:52:20.500
- I'll just add down in California a couple years ago,

00:52:20.500 --> 00:52:23.100
we had a very delayed crab season because of a

00:52:23.100 --> 00:52:26.820
toxic algal bloom, so that was another effect of

00:52:27.460 --> 00:52:30.340
changing ocean conditions, a very late toxic algal

00:52:30.340 --> 00:52:33.580
bloom that they had to delay crab season significantly,

00:52:33.580 --> 00:52:37.500
which really hurts the communities of folks that

00:52:37.500 --> 00:52:41.160
rely on catching crab.

00:52:41.680 --> 00:52:44.540
- Alright, we have a note from an ambitious young

00:52:44.540 --> 00:52:47.600
man, who's saying, "Hi, Dr. Trigg, can ocean

00:52:47.600 --> 00:52:50.220
acidification be generalized across crustacean

00:52:50.220 --> 00:52:53.380
species, and are there differences in bays and

00:52:53.380 --> 00:52:55.540
open ocean in terms of carbon intake and

00:52:55.540 --> 00:52:57.140
associated pH levels?"

00:52:57.140 --> 00:52:59.580
And on a side note, he's wondering if you're

00:52:59.580 --> 00:53:02.840
look for an intern. I don't know where he's based, but

00:53:02.840 --> 00:53:05.760
he's being ambitious here.

00:53:05.920 --> 00:53:07.860
- So, for the internships and stuff like that, you

00:53:07.860 --> 00:53:11.480
should definitely check out the NOAA website,

00:53:11.820 --> 00:53:16.120
the NOAA ocean acidification program, and look for

00:53:16.180 --> 00:53:17.860
opportunities that way.

00:53:18.200 --> 00:53:20.920
I'm currently at University of Washington now, I just

00:53:20.920 --> 00:53:24.080
started here, so not looking for interns yet, but

00:53:24.080 --> 00:53:27.740
maybe some time in the future.

00:53:27.740 --> 00:53:30.300
And then, so we're thinking about...

00:53:30.300 --> 00:53:33.420
- The first part of the question is ocean acidification

00:53:33.420 --> 00:53:36.300
generalized across crustacean species?

00:53:37.460 --> 00:53:44.340
- So, I don't think we know that, we only kind of

00:53:44.340 --> 00:53:49.560
know what we've investigated, the dungeness crab

00:53:49.560 --> 00:53:53.740
response to, I don't know this is kind of different,

00:53:53.740 --> 00:53:56.840
low oxygen was a response that is conserved

00:53:56.840 --> 00:53:59.060
across different animals when they're in low oxygen

00:53:59.060 --> 00:54:00.940
conditions, so that is something that could be

00:54:00.940 --> 00:54:05.280
generalized, but I don't know about the ocean

00:54:05.280 --> 00:54:09.060
acidification response. I mean that has to do with

00:54:09.060 --> 00:54:12.500
kind of how the animal prioritizes which functions

00:54:12.720 --> 00:54:15.000
are important, kind of like the second question that

00:54:15.000 --> 00:54:17.900
we all thought about, and that's something that

00:54:17.960 --> 00:54:21.080
might be different, depending on the species.

00:54:22.760 --> 00:54:28.600
So, I'm not sure, and definitely bays versus offshore,

00:54:28.600 --> 00:54:33.360
off the coast, I think vary in pH, it's kind of

00:54:33.360 --> 00:54:37.760
dependent on how much river water is flowing into

00:54:38.160 --> 00:54:42.200
that and there's a lot of different variables that can

00:54:42.200 --> 00:54:44.120
change the water chemistry.

00:54:44.920 --> 00:54:48.360
- Alright, excellent. So we did have someone mention

00:54:48.360 --> 00:54:50.980
that on NOAA's website under the teacher curriculum,

00:54:50.980 --> 00:54:53.900
there's a space for O.A. curriculum coming soon, and

00:54:53.920 --> 00:54:56.380
so wanting to know more about that.

00:54:56.380 --> 00:54:58.220
And I also just wanted to just mention that initially

00:54:58.220 --> 00:55:00.380
today, on October 17th, we were going to have a

00:55:00.380 --> 00:55:03.920
presentation by Amy Dean talking about our real

00:55:03.920 --> 00:55:07.620
time data or near real time data in our NOAA data

00:55:07.620 --> 00:55:10.640
in the classroom resource on ocean acidification,

00:55:11.000 --> 00:55:13.800
and unfortunately those materials haven't yet been

00:55:13.800 --> 00:55:16.780
solidified and put onto the internet, so hence why

00:55:16.780 --> 00:55:20.220
we had to cancel that particular webinar, but there

00:55:20.600 --> 00:55:24.600
is more educational material on ocean acidification

00:55:24.600 --> 00:55:27.180
coming out from different parts of NOAA, like the

00:55:27.180 --> 00:55:29.280
Office of National Marine Sanctuaries and the

00:55:29.280 --> 00:55:32.340
ocean acidification program, and of course this

00:55:32.340 --> 00:55:35.660
collaboration with NOAA fisheries, so keep

00:55:35.800 --> 00:55:37.820
checking back because some of those curriculum

00:55:37.820 --> 00:55:40.940
materials and other educational complements

00:55:41.220 --> 00:55:43.460
will be available soon.

00:55:43.740 --> 00:55:46.580
Okay, we can ask maybe one more question, and

00:55:46.580 --> 00:55:49.060
there's so many to choose from, so...

00:55:51.560 --> 00:55:55.540
Okay, that's similar, oh goodness, okay.

00:55:55.580 --> 00:55:58.380
- While you're doing that, I just want to add that

00:55:58.380 --> 00:56:01.220
cleanet.org is a really great curriculum resource

00:56:01.260 --> 00:56:04.080
for searching for curriculum related to climate,

00:56:04.160 --> 00:56:06.200
and you can search ocean acidification. It's supported

00:56:06.200 --> 00:56:09.180
by NOAA, and it's all curriculum that's reviewed by

00:56:09.180 --> 00:56:13.740
scientists for accuracy and also the pedagogy for

00:56:13.740 --> 00:56:18.240
the standards, so cleanet.org is a really great

00:56:18.240 --> 00:56:21.440
curriculum resource.

00:56:22.600 --> 00:56:25.800
- So, this kind of goes back again to comparing

00:56:25.800 --> 00:56:28.320
crabs and other shellfish and such but can we

00:56:28.320 --> 00:56:31.620
assume that bivalves will have a more difficult time

00:56:31.620 --> 00:56:35.140
making their calcium carbonate shells than animals

00:56:35.280 --> 00:56:38.000
like crabs that use chitin for shells with

00:56:38.040 --> 00:56:41.820
ocean acidification? And your response to that, Shelly.

00:56:44.540 --> 00:56:49.660
- I think in terms of shells that bivalve shells are

00:56:49.780 --> 00:56:55.520
more vulnerable to dissolution than chitin, but there

00:56:55.680 --> 00:56:59.800
may be other processes in the dungeness crab that

00:56:59.800 --> 00:57:03.260
get compromised that might not get compromised

00:57:03.400 --> 00:57:07.440
in bivalves, and it's those kinds of underlying, so

00:57:07.440 --> 00:57:12.240
besides the shell, what other biology is affected,

00:57:12.460 --> 00:57:20.400
and how bad of a problem is it? Those are all things

00:57:20.400 --> 00:57:24.240
that we're trying to look at.

00:57:24.800 --> 00:57:28.200
- Okay, well with that, any remaining questions that

00:57:28.200 --> 00:57:31.020
haven't been answered, we'll go ahead and I'll email

00:57:31.020 --> 00:57:33.980
those to Shelly and in the next week or so, we'll get

00:57:33.980 --> 00:57:35.800
a response back to all attendees.

00:57:35.800 --> 00:57:40.220
So, I did put a link into the box there in your control

00:57:40.220 --> 00:57:43.180
panel for the ocean acidification toolkit.

00:57:44.280 --> 00:57:47.000
Jenny has also provided links for the NOAA ocean

00:57:47.000 --> 00:57:50.440
acidification program, that cleanet.org.

00:57:50.720 --> 00:57:53.360
There's also a lot of great resources available

00:57:53.900 --> 00:57:57.600
on the Understanding Ocean Acidification website,

00:57:58.200 --> 00:57:59.780
that I put a link in there, it's

00:57:59.780 --> 00:58:01.780
cisanctuary.org/acidocean.

00:58:02.940 --> 00:58:05.040
And so thanks to many of you that when we were

00:58:05.040 --> 00:58:07.100
having the discussion about teaching

00:58:07.100 --> 00:58:09.840
ocean acidification to elementary school students,

00:58:09.840 --> 00:58:12.860
a lot of you wrote in ideas of how to demonstrate

00:58:12.960 --> 00:58:15.320
the process, so appreciate that.

00:58:15.320 --> 00:58:17.900
There are some good ideas, which are similar to

00:58:17.900 --> 00:58:20.940
what many of you wrote on that acid ocean website.

00:58:21.420 --> 00:58:24.960
With that, I'll go ahead and take back the controls

00:58:24.960 --> 00:58:27.200
to wrap up today's presentation.

00:58:28.340 --> 00:58:34.900
So, let's see here, okay, thanks again, Shelly, for

00:58:35.000 --> 00:58:38.080
a great presentation, we appreciate your time being

00:58:38.080 --> 00:58:41.820
on today's call. I mentioned earlier that this webinar

00:58:41.820 --> 00:58:44.780
has been recorded and there is an archive page,

00:58:44.900 --> 00:58:47.800
there's no need to do a mad rush and write down

00:58:47.800 --> 00:58:50.340
that URL because it will be coming to you in a

00:58:50.340 --> 00:58:51.480
follow-up email.

00:58:51.480 --> 00:58:53.700
If you do have any specific questions or comments,

00:58:53.700 --> 00:58:58.500
go ahead and email sanctuary.education@noaa.gov.

00:58:58.860 --> 00:59:01.420
Based on feedback a couple years ago when we

00:59:01.420 --> 00:59:04.000
first started this webinar series, we now create a

00:59:04.000 --> 00:59:07.360
certificate of attendance, so all attendees today

00:59:07.360 --> 00:59:10.680
in the live presentation will receive this certificate

00:59:10.680 --> 00:59:13.880
that is showing one contact hour of professional

00:59:13.880 --> 00:59:17.240
development and you'll be getting that just in the

00:59:17.300 --> 00:59:20.500
few minutes here following the webinar via email.

00:59:20.500 --> 00:59:23.140
I normally like to advertise the next webinar in the

00:59:23.140 --> 00:59:26.520
series, but we are taking a hiatus for November.

00:59:26.520 --> 00:59:29.860
I think our website still shows that there is a

00:59:29.860 --> 00:59:32.940
Sinkholes in Thunder Bay Sanctuary presentation,

00:59:32.940 --> 00:59:35.640
but unfortunately that has to be cancelled , so

00:59:35.640 --> 00:59:38.340
November is off and then we'll start back in

00:59:38.340 --> 00:59:40.740
December with another presentation that is being

00:59:40.740 --> 00:59:43.900
finalized now, and as soon as possible, I'll get the

00:59:43.900 --> 00:59:48.540
calendar of events for the presentations for 2019

00:59:49.080 --> 00:59:51.080
up on our website.

00:59:51.080 --> 00:59:53.560
And lastly, I mentioned this in the beginning, but

00:59:53.560 --> 00:59:56.160
before people sign off, when you do close out from

00:59:56.160 --> 00:59:59.520
the webinar, make sure to just please spend those

00:59:59.520 --> 01:00:02.340
two or three minutes answering our questions.

01:00:02.340 --> 01:00:05.740
We take this evaluation data very seriously, we

01:00:05.820 --> 01:00:09.500
analyze all of it, and find ways to improve the series

01:00:09.500 --> 01:00:12.560
and some of our other educational opportunities.

01:00:12.560 --> 01:00:15.060
For those of you that are educators, we do ask a

01:00:15.060 --> 01:00:17.560
series of questions about bringing NOAA science

01:00:17.620 --> 01:00:20.600
into your classroom, where it's similar to a webinar,

01:00:20.600 --> 01:00:23.460
but we would do it with you as a teacher and your

01:00:23.480 --> 01:00:26.340
students, and so we are doing a needs assessment

01:00:26.340 --> 01:00:29.640
on what kind of platform and what we would do

01:00:29.640 --> 01:00:31.780
for that, so if you're an educator, feel free to answer

01:00:31.860 --> 01:00:34.020
those following questions as well.

01:00:34.740 --> 01:00:36.860
With that, we will end today's webinar, but we

01:00:36.860 --> 01:00:39.920
appreciate all of your time, for participating, and

01:00:39.920 --> 01:00:42.760
Doctor Shelly Trigg for joining us and Jennifer Stock

01:00:42.800 --> 01:00:46.380
up at Point Reyes, appreciate everyone and with

01:00:46.380 --> 01:00:49.580
that, it concludes today's webinar. Thank you.

