WEBVTT 1 00:00:00.000 --> 00:00:05.000 Everyone to our National Marine Sanctuaries 2 00:00:05.910 --> 00:00:08.160 Webinar Series. 3 00:00:08.160 --> 00:00:12.090 We're so pleased to have you all joining us today 4 00:00:12.090 --> 00:00:17.090 for our special presentation on Seagrass Wasting syndrome. 5 00:00:18.090 --> 00:00:21.870 So I wanted to kick off by letting you all know 6 00:00:21.870 --> 00:00:26.610 that as attendees, you'll be joining us in listen only mode. 7 00:00:26.610 --> 00:00:28.410 If you have any questions, 8 00:00:28.410 --> 00:00:33.000 you can type them into the GoToWebinar question box, 9 00:00:33.000 --> 00:00:37.170 and we'll get to those either through a typed response 10 00:00:37.170 --> 00:00:39.333 or during our Q&A section. 11 00:00:40.500 --> 00:00:43.950 If you have any audio or visual trouble, 12 00:00:43.950 --> 00:00:46.860 please go ahead and also type that into the question box 13 00:00:46.860 --> 00:00:49.830 and we will do our best to try to help you 14 00:00:49.830 --> 00:00:53.850 navigate some of those AV issues. 15 00:00:53.850 --> 00:00:57.873 So with that, let's go ahead and get started. 16 00:00:59.160 --> 00:01:04.080 Today, myself and Brian Chansky will be hosts. 17 00:01:04.080 --> 00:01:07.710 I'm Claire Fackler. I go by she/her pronouns. 18 00:01:07.710 --> 00:01:12.060 I'm the National Education Liaison of the NOAA Office 19 00:01:12.060 --> 00:01:14.760 of National Marine Sanctuaries, 20 00:01:14.760 --> 00:01:16.380 and I'm joined by Brian, 21 00:01:16.380 --> 00:01:21.000 who is a virtual student federal service e-intern 22 00:01:21.000 --> 00:01:24.423 who's a undergraduate at the University of Delaware. 23 00:01:25.470 --> 00:01:27.840 So we welcome all of you. 24 00:01:27.840 --> 00:01:30.930 As usual, we'd like to do just a few intro slides 25 00:01:30.930 --> 00:01:32.760 to kind of set the stage 26 00:01:32.760 --> 00:01:37.260 of where this science and research is taking place. 27 00:01:37.260 --> 00:01:39.900 So this you're looking at 28 00:01:39.900 --> 00:01:43.560 is the National Marine Sanctuary System. 29 00:01:43.560 --> 00:01:46.710 You can think of it like the National Park System, 30 00:01:46.710 --> 00:01:49.890 but these are basically underwater parks. 31 00:01:49.890 --> 00:01:53.250 So each of these white dots on the map represent 32 00:01:53.250 --> 00:01:56.070 one of our national marine sanctuaries 33 00:01:56.070 --> 00:01:59.940 or special ocean or Great Lakes treasures. 34 00:01:59.940 --> 00:02:02.460 We have some triangles in the South Pacific 35 00:02:02.460 --> 00:02:04.980 and the northwestern Hawaiian islands 36 00:02:04.980 --> 00:02:06.660 that are also underwater parks. 37 00:02:06.660 --> 00:02:09.480 They're just called marine national monuments. 38 00:02:09.480 --> 00:02:12.960 So different terminology, but basically the same thing. 39 00:02:12.960 --> 00:02:15.750 And then we've got these yellow boxes. 40 00:02:15.750 --> 00:02:16.920 That's really exciting 41 00:02:16.920 --> 00:02:19.650 because during President Biden's 42 00:02:19.650 --> 00:02:22.530 America the Beautiful initiative which aligns 43 00:02:22.530 --> 00:02:27.530 with the global 30 by 30 is an effort to expand public lands 44 00:02:29.430 --> 00:02:34.430 and public waters by 30% by the year 2030. 45 00:02:34.800 --> 00:02:38.610 So a big time of expansion globally 46 00:02:38.610 --> 00:02:43.590 as well as in the United States in the sanctuary system. 47 00:02:43.590 --> 00:02:45.570 So all said, right now, 48 00:02:45.570 --> 00:02:50.310 NOAA manages about 620,000 square miles 49 00:02:50.310 --> 00:02:52.200 of ocean and Great Lakes treasures 50 00:02:52.200 --> 00:02:54.783 that we're protecting now and for the future. 51 00:02:55.740 --> 00:02:58.260 And these treasures are set aside 52 00:02:58.260 --> 00:03:01.590 for a wide variety of reasons, often by Congress, 53 00:03:01.590 --> 00:03:05.010 but through a stakeholder-driven process. 54 00:03:05.010 --> 00:03:07.920 In some cases, our marine sanctuaries 55 00:03:07.920 --> 00:03:10.950 and marine national monuments are set aside 56 00:03:10.950 --> 00:03:13.773 because of the incredible biodiversity. 57 00:03:14.640 --> 00:03:17.820 In other cases, it's because of the shipwrecks 58 00:03:17.820 --> 00:03:20.493 or cultural or maritime heritage. 59 00:03:21.630 --> 00:03:25.890 But all in all, these sites help provide shelter 60 00:03:25.890 --> 00:03:28.650 for threatened and endangered marine life, 61 00:03:28.650 --> 00:03:30.753 like this Hawaiian Green Sea turtle. 62 00:03:31.650 --> 00:03:34.530 So Congress actually mandates NOAA 63 00:03:34.530 --> 00:03:37.710 and the Office of National Marine Sanctuaries 64 00:03:37.710 --> 00:03:42.710 to conduct education and outreach. 65 00:03:44.160 --> 00:03:49.160 We're mandated to do research and monitoring, 66 00:03:49.800 --> 00:03:53.640 and all of this with the intent of protecting the resource, 67 00:03:53.640 --> 00:03:57.753 so protecting the habitat, the ecosystem. 68 00:03:58.920 --> 00:04:01.020 And I've already mentioned national parks. 69 00:04:01.020 --> 00:04:05.280 So you know that those land-based parks 70 00:04:05.280 --> 00:04:09.000 that are in the National Park system are special areas. 71 00:04:09.000 --> 00:04:12.360 So these are the special ocean and Great Lakes places. 72 00:04:12.360 --> 00:04:13.680 And just like a national park, 73 00:04:13.680 --> 00:04:17.129 we want you to get into it and explore and enjoy 74 00:04:17.129 --> 00:04:20.250 and fall in love with these special ocean areas 75 00:04:20.250 --> 00:04:22.500 and Great Lakes places. 76 00:04:22.500 --> 00:04:25.740 So there's so many different activities that you can do 77 00:04:25.740 --> 00:04:29.580 in your special sanctuaries and monuments. 78 00:04:29.580 --> 00:04:30.783 You can kayak. 79 00:04:31.680 --> 00:04:35.583 In some cases, you can fish, you can snorkel, 80 00:04:36.420 --> 00:04:39.273 scuba dive, surf, 81 00:04:40.320 --> 00:04:43.530 get on a boat, view marine life. 82 00:04:43.530 --> 00:04:45.870 Oftentimes, when people get to interact 83 00:04:45.870 --> 00:04:47.700 with these special ocean treasures, 84 00:04:47.700 --> 00:04:49.320 they get so inspired 85 00:04:49.320 --> 00:04:52.740 that they actually become volunteers for us. 86 00:04:52.740 --> 00:04:57.210 So with that, I will let Brian introduce our presenter 87 00:04:57.210 --> 00:04:58.410 who's going to be zooming in 88 00:04:58.410 --> 00:05:01.770 to our Greater Farallones National Marine sanctuary 89 00:05:01.770 --> 00:05:03.453 talking about seagrass. 90 00:05:05.610 --> 00:05:07.273 Thank you, Claire. 91 00:05:07.273 --> 00:05:10.920 All right, Serina Moheed is a second year PhD student 92 00:05:10.920 --> 00:05:13.260 at the University of California, Davis 93 00:05:13.260 --> 00:05:15.720 studying seagrass wasting disease 94 00:05:15.720 --> 00:05:17.880 in Northern California estuaries. 95 00:05:17.880 --> 00:05:20.730 As a NOAA, Nancy Foster scholar, she partners 96 00:05:20.730 --> 00:05:23.520 with the Greater Farallones National Marine Sanctuary 97 00:05:23.520 --> 00:05:27.810 to identify seagrass populations vulnerable to the disease 98 00:05:27.810 --> 00:05:31.560 and thinks about how to encourage resilience in these areas. 99 00:05:31.560 --> 00:05:32.850 Serina, take it away. 100 00:05:36.420 --> 00:05:37.500 And Serena while... 101 00:05:37.500 --> 00:05:39.780 Oh good. I was gonna say we wanna see you and there you are. 102 00:05:39.780 --> 00:05:41.130 So we're gonna pop off, Brian, 103 00:05:41.130 --> 00:05:43.530 and we'll let Serina take the center stage here. 104 00:05:45.630 --> 00:05:47.493 All right, perfect. Let's see. 105 00:05:55.200 --> 00:05:58.710 Sorry, just getting everything set up. 106 00:05:58.710 --> 00:06:00.240 Looks great. 107 00:06:00.240 --> 00:06:02.193 Oh, looks good? Okay, awesome. 108 00:06:03.300 --> 00:06:05.100 So you can see my screen okay, then? 109 00:06:06.690 --> 00:06:07.977 Yes, we can. 110 00:06:07.977 --> 00:06:12.210 All right, perfect. All right. 111 00:06:12.210 --> 00:06:13.260 Hello, everyone. 112 00:06:13.260 --> 00:06:16.320 Thank you, Brian and Claire, for the wonderful introduction, 113 00:06:16.320 --> 00:06:18.150 and thank you all for joining me today. 114 00:06:18.150 --> 00:06:22.140 So I'm gonna be talking about how plants get sick too, 115 00:06:22.140 --> 00:06:24.570 and monitoring seagrass wasting disease 116 00:06:24.570 --> 00:06:26.133 in a changing climate. 117 00:06:29.880 --> 00:06:32.040 So I first just want to run through a little bit more 118 00:06:32.040 --> 00:06:32.970 of an introduction. 119 00:06:32.970 --> 00:06:36.060 So again, hello, I'm Serina. 120 00:06:36.060 --> 00:06:37.830 I'm a second year PhD student 121 00:06:37.830 --> 00:06:40.170 at the University of California, Davis, 122 00:06:40.170 --> 00:06:42.690 and I study seagrass wasting disease. 123 00:06:42.690 --> 00:06:44.604 I'm also honored to be 124 00:06:44.604 --> 00:06:47.760 a Dr. Nancy Foster scholar and partner 125 00:06:47.760 --> 00:06:50.430 with the Greater Farallones National Marine Sanctuary 126 00:06:50.430 --> 00:06:52.740 to help protect our seagrass beds. 127 00:06:52.740 --> 00:06:54.900 And I'm super excited to talk with you today 128 00:06:54.900 --> 00:06:56.373 about marine diseases. 129 00:06:59.370 --> 00:07:02.550 So just to go through a brief outline 130 00:07:02.550 --> 00:07:04.440 of what we're gonna be talking about today, 131 00:07:04.440 --> 00:07:08.477 I'm gonna introduce you guys to what our marine disease is, 132 00:07:08.477 --> 00:07:10.260 what causes them, 133 00:07:10.260 --> 00:07:15.240 how can these be displayed in different organisms. 134 00:07:15.240 --> 00:07:18.000 And then kind of zooming into my study species. 135 00:07:18.000 --> 00:07:21.510 So what are seagrasses and why are they important? 136 00:07:21.510 --> 00:07:25.980 And then also then talking about seagrass wasting disease. 137 00:07:25.980 --> 00:07:28.740 So what is it and how do I study it 138 00:07:28.740 --> 00:07:31.083 and how am I using it for my PhD? 139 00:07:33.750 --> 00:07:36.060 So in the last couple of years, 140 00:07:36.060 --> 00:07:38.070 you might have seen some headlines 141 00:07:38.070 --> 00:07:39.753 that look a bit like this. 142 00:07:40.740 --> 00:07:44.760 And so marine diseases are caused by pathogens in the ocean 143 00:07:44.760 --> 00:07:46.470 that infect marine organisms, 144 00:07:46.470 --> 00:07:49.080 and it's actually much more common than you might think. 145 00:07:49.080 --> 00:07:54.080 And so they can affect coral reefs, starfish, oysters, 146 00:07:54.480 --> 00:07:58.590 and even larger marine mammals like sea otters. 147 00:07:58.590 --> 00:08:00.900 And so disease outbreaks can occur suddenly 148 00:08:00.900 --> 00:08:04.380 and without warning and infect many individuals 149 00:08:04.380 --> 00:08:06.030 in a short amount of time. 150 00:08:06.030 --> 00:08:08.250 And because there's often more than one cause 151 00:08:08.250 --> 00:08:10.950 for why a disease outbreak occurs, 152 00:08:10.950 --> 00:08:13.020 scientists are still kind of unraveling 153 00:08:13.020 --> 00:08:16.500 the who, what, where, when, and why 154 00:08:16.500 --> 00:08:18.273 around a lot of these diseases. 155 00:08:20.820 --> 00:08:23.580 And marine diseases can take on many forms 156 00:08:23.580 --> 00:08:25.500 and have different visual symptoms, 157 00:08:25.500 --> 00:08:27.570 much like terrestrial diseases. 158 00:08:27.570 --> 00:08:31.350 So some organisms can show lesions or tissue loss 159 00:08:31.350 --> 00:08:33.840 such as with stony coral tissue disease 160 00:08:33.840 --> 00:08:35.400 and sea star wasting disease, 161 00:08:35.400 --> 00:08:36.810 which you might've heard of. 162 00:08:36.810 --> 00:08:39.960 And this is actually where sea star limbs 163 00:08:39.960 --> 00:08:41.793 will detach from their body. 164 00:08:42.900 --> 00:08:45.450 But there also might be more kind of internal symptoms 165 00:08:45.450 --> 00:08:49.110 such as muscle loss with oysters herpesvirus, 166 00:08:49.110 --> 00:08:53.070 and brain inflammation with sea otter toxoplasmosis. 167 00:08:53.070 --> 00:08:55.230 So you can see that there's a range of symptoms 168 00:08:55.230 --> 00:08:57.480 that can occur with marine diseases. 169 00:08:57.480 --> 00:09:00.870 And so it's really important that we know what these are 170 00:09:00.870 --> 00:09:02.253 and what to look out for. 171 00:09:05.010 --> 00:09:09.420 And to help us tease apart why disease outbreaks occur, 172 00:09:09.420 --> 00:09:12.030 disease ecology uses a conceptual diagram 173 00:09:12.030 --> 00:09:13.710 called the disease triangle, 174 00:09:13.710 --> 00:09:15.960 and that's really meant to show the three main players 175 00:09:15.960 --> 00:09:19.770 necessary for any infectious disease to spread. 176 00:09:19.770 --> 00:09:22.770 And this also applies to terrestrial diseases. 177 00:09:22.770 --> 00:09:24.780 So we can use COVID as an example 178 00:09:24.780 --> 00:09:27.180 that we are all familiar with. 179 00:09:27.180 --> 00:09:30.600 So at the start of the COVID pandemic, we had our pathogen, 180 00:09:30.600 --> 00:09:31.863 the coronavirus. 181 00:09:32.760 --> 00:09:36.033 We also had hosts that were susceptible to this pathogen. 182 00:09:36.900 --> 00:09:38.730 And then we had an environment where people 183 00:09:38.730 --> 00:09:40.110 were interacting with each other 184 00:09:40.110 --> 00:09:41.730 to spread the pathogen around. 185 00:09:41.730 --> 00:09:45.603 And all these together created a disease pandemic. 186 00:09:46.920 --> 00:09:50.820 And to protect, especially vulnerable populations, 187 00:09:50.820 --> 00:09:52.440 scientists and doctors were recommending 188 00:09:52.440 --> 00:09:55.080 that people socially distance to try to stop the spread 189 00:09:55.080 --> 00:09:58.680 between hosts in a time where no one was resistant 190 00:09:58.680 --> 00:10:01.350 to this disease. 191 00:10:01.350 --> 00:10:02.430 And then at the same time, 192 00:10:02.430 --> 00:10:05.430 the pathogen itself was also evolving, 193 00:10:05.430 --> 00:10:09.000 but some of these new strains couldn't infect as many people 194 00:10:09.000 --> 00:10:10.530 because as a population, 195 00:10:10.530 --> 00:10:14.100 we had developed immunity through either vaccination 196 00:10:14.100 --> 00:10:16.473 or naturally recovering from the disease. 197 00:10:17.370 --> 00:10:21.540 But if your pathogen is in the water, 198 00:10:21.540 --> 00:10:23.520 how do you implement social distancing 199 00:10:23.520 --> 00:10:25.380 when your organism can't move 200 00:10:25.380 --> 00:10:28.623 and it's constantly exposed to this infectious environment? 201 00:10:29.640 --> 00:10:32.670 How do you figure out if the pathogen is evolving 202 00:10:32.670 --> 00:10:35.763 and whether host populations are resistant to it? 203 00:10:36.690 --> 00:10:39.960 So these are the kinds of questions 204 00:10:39.960 --> 00:10:42.150 that marine disease ecologists encounter 205 00:10:42.150 --> 00:10:44.190 when faced with an epidemic. 206 00:10:44.190 --> 00:10:46.620 And similarly, the same types of questions 207 00:10:46.620 --> 00:10:49.383 that I'm thinking about with my research on seagrasses. 208 00:10:52.230 --> 00:10:54.240 So just to start us off, 209 00:10:54.240 --> 00:10:56.160 I want to ask a little bit of poll question. 210 00:10:56.160 --> 00:10:58.980 So I wanna know how many of you guys know 211 00:10:58.980 --> 00:11:00.303 what seagrasses are? 212 00:11:02.340 --> 00:11:03.210 All right, everyone, 213 00:11:03.210 --> 00:11:05.130 the poll question has been launched, 214 00:11:05.130 --> 00:11:08.310 so go ahead and put in your response. 215 00:11:08.310 --> 00:11:11.133 And we are clicking up the votes here. 216 00:11:12.900 --> 00:11:17.043 This is a good way to assess the knowledge of our audience. 217 00:11:20.970 --> 00:11:23.700 All right, we're inching to 80% of you voting, 218 00:11:23.700 --> 00:11:25.950 so voting's important. 219 00:11:25.950 --> 00:11:29.970 Couple more seconds. (Serina chuckles) 220 00:11:29.970 --> 00:11:33.090 All right, go ahead and share our results. 221 00:11:33.090 --> 00:11:38.043 So 95% of our audience know what seagrass is. 222 00:11:39.420 --> 00:11:42.523 We've got a very knowledgeable audience here with us. 223 00:11:42.523 --> 00:11:44.640 (Serina chuckles) 224 00:11:44.640 --> 00:11:46.413 All right. That's amazing. 225 00:11:47.670 --> 00:11:50.100 Okay, are you guys still seeing my screen 226 00:11:50.100 --> 00:11:51.540 or do I need to share it again? 227 00:11:51.540 --> 00:11:53.310 No, we are. You're back on. 228 00:11:53.310 --> 00:11:56.220 Oh, perfect. Okay. 229 00:11:56.220 --> 00:11:59.070 So that's really awesome. 230 00:11:59.070 --> 00:12:00.570 I was not expecting that, 231 00:12:00.570 --> 00:12:02.640 but it also makes me so happy that so many of you know 232 00:12:02.640 --> 00:12:03.990 what seagrasses are. 233 00:12:03.990 --> 00:12:05.940 So some of this might be reviewed for you guys, 234 00:12:05.940 --> 00:12:09.390 but hopefully you do end up learning something 235 00:12:09.390 --> 00:12:10.773 from this presentation. 236 00:12:11.760 --> 00:12:14.010 So a little bit of background on the folks 237 00:12:14.010 --> 00:12:16.500 that are not as familiar with seagrasses. 238 00:12:16.500 --> 00:12:19.890 So seagrasses are marine flowering plants 239 00:12:19.890 --> 00:12:22.380 that grow in salty and brackish waters. 240 00:12:22.380 --> 00:12:25.110 So this is water usually found in estuaries 241 00:12:25.110 --> 00:12:27.903 where salt water is mixing with fresh water, 242 00:12:28.740 --> 00:12:30.540 and they usually grow pretty shallow, 243 00:12:30.540 --> 00:12:32.670 so around one to three meters deep. 244 00:12:32.670 --> 00:12:35.400 But of course they have been found a lot deeper 245 00:12:35.400 --> 00:12:40.400 and there are plenty of deeper seagrass beds. 246 00:12:40.410 --> 00:12:42.570 And there was a recent assessment that concluded 247 00:12:42.570 --> 00:12:45.030 if you gather all the seagrass in the world, 248 00:12:45.030 --> 00:12:49.530 it will cover an area of about 160,000 kilometers squared, 249 00:12:49.530 --> 00:12:52.440 which to put that in context is about the size 250 00:12:52.440 --> 00:12:54.240 of the state of Georgia. 251 00:12:54.240 --> 00:12:58.650 However, seagrass is mapped very poorly. 252 00:12:58.650 --> 00:13:01.980 And so some people estimate that the current number 253 00:13:01.980 --> 00:13:03.990 actually might be three times this size, 254 00:13:03.990 --> 00:13:06.663 which would be about the size of France. 255 00:13:08.130 --> 00:13:12.570 And so the picture in the middle is a diagram showing you 256 00:13:12.570 --> 00:13:14.910 what seagrass plant would look like. 257 00:13:14.910 --> 00:13:19.740 And so like tree glasses, they have blades and leaves. 258 00:13:19.740 --> 00:13:20.910 And unlike seaweeds, 259 00:13:20.910 --> 00:13:23.310 they have this kind of root and rhizome system 260 00:13:23.310 --> 00:13:26.793 that connects them to other plants underneath the ground. 261 00:13:28.110 --> 00:13:32.790 And they reproduce asexually by producing clones. 262 00:13:32.790 --> 00:13:34.860 So you can think that if you're trying to propagate a plant, 263 00:13:34.860 --> 00:13:36.450 you take a cutting and then that'll grow 264 00:13:36.450 --> 00:13:38.970 into a whole new plant by itself. 265 00:13:38.970 --> 00:13:42.120 And then they also produce sexually via pollination 266 00:13:42.120 --> 00:13:43.470 and producing seeds. 267 00:13:43.470 --> 00:13:46.110 So the flower in that diagram actually looks 268 00:13:46.110 --> 00:13:49.770 a lot more like the picture to the far right there. 269 00:13:49.770 --> 00:13:53.520 So that is a flowering blade of seagrass. 270 00:13:53.520 --> 00:13:56.760 And those oblong shapes are actually fertilized seeds 271 00:13:56.760 --> 00:14:01.590 that are ready to drop off and grow new seagrass plants. 272 00:14:01.590 --> 00:14:03.030 And so in this way, 273 00:14:03.030 --> 00:14:05.250 seagrasses can form kind of dense patches 274 00:14:05.250 --> 00:14:07.950 that will eventually grow to entire meadows 275 00:14:07.950 --> 00:14:11.043 that can sometimes be miles or kilometers long. 276 00:14:14.160 --> 00:14:16.140 And so where are seagrasses found? 277 00:14:16.140 --> 00:14:18.840 So they are found globally and are probably more common 278 00:14:18.840 --> 00:14:20.160 than you might imagine. 279 00:14:20.160 --> 00:14:22.290 And so this world map is showing 280 00:14:22.290 --> 00:14:23.820 where seagrasses are present. 281 00:14:23.820 --> 00:14:26.400 And so the darker colors are representing 282 00:14:26.400 --> 00:14:30.360 places with highest seagrass abundance. 283 00:14:30.360 --> 00:14:33.450 And you can see that essentially all the continents 284 00:14:33.450 --> 00:14:35.850 except for Antarctica have seagrass as present 285 00:14:35.850 --> 00:14:38.460 in some capacity on their coasts. 286 00:14:38.460 --> 00:14:40.620 And the darker colors here also correspond 287 00:14:40.620 --> 00:14:45.020 with areas where there are multiple species 288 00:14:45.020 --> 00:14:46.473 of seagrasses present. 289 00:14:47.340 --> 00:14:49.620 And so seagrasses can kinda be thought of 290 00:14:49.620 --> 00:14:51.780 as the grasslands of the sea. 291 00:14:51.780 --> 00:14:53.520 They might not be that important, 292 00:14:53.520 --> 00:14:57.030 but they do harbor an incredible amount of biodiversity. 293 00:14:57.030 --> 00:15:00.930 And I did have some videos queued up to share with you guys, 294 00:15:00.930 --> 00:15:03.630 but unfortunately technology was not on our side today. 295 00:15:03.630 --> 00:15:06.780 And so instead, I am just gonna tell you that 296 00:15:06.780 --> 00:15:11.730 you can have a range of organisms in seagrass beds 297 00:15:11.730 --> 00:15:14.070 from really tiny to really large. 298 00:15:14.070 --> 00:15:15.810 And so you can get surfperch 299 00:15:15.810 --> 00:15:17.340 that are eating the tiny invertebrates 300 00:15:17.340 --> 00:15:19.620 that live along the seagrass. 301 00:15:19.620 --> 00:15:22.080 You could have ducks that actually feed 302 00:15:22.080 --> 00:15:24.000 on the seagrass itself. 303 00:15:24.000 --> 00:15:26.760 And you can also have larger organisms 304 00:15:26.760 --> 00:15:29.643 such as leopard sharks and harbor seals. 305 00:15:31.560 --> 00:15:35.280 And so there's a number of organisms 306 00:15:35.280 --> 00:15:37.770 that actually use the seagrass habitat 307 00:15:37.770 --> 00:15:41.133 or use the seagrass as habitat and then also for food. 308 00:15:43.110 --> 00:15:45.980 So that brings us to why are seagrasses important. 309 00:15:45.980 --> 00:15:47.130 So we just kind of covered 310 00:15:47.130 --> 00:15:50.070 that they can be a foundational ecosystem 311 00:15:50.070 --> 00:15:54.180 that's home to many different species, both large and small. 312 00:15:54.180 --> 00:15:57.510 And given that meadows are often found in shallower water, 313 00:15:57.510 --> 00:15:59.700 they can actually decrease wave energy 314 00:15:59.700 --> 00:16:01.893 and protect coastlines from storms. 315 00:16:03.480 --> 00:16:06.060 They can also store carbon from the atmosphere 316 00:16:06.060 --> 00:16:07.080 into their sediment. 317 00:16:07.080 --> 00:16:08.820 And so we actually refer to these systems 318 00:16:08.820 --> 00:16:10.950 as blue carbon systems, 319 00:16:10.950 --> 00:16:13.260 and possibly more importantly, 320 00:16:13.260 --> 00:16:15.120 they produce the oxygen that we breathe. 321 00:16:15.120 --> 00:16:18.630 And so it's estimated that one square meter of seagrass 322 00:16:18.630 --> 00:16:21.933 can actually produce up to 10 liters of oxygen a day. 323 00:16:23.820 --> 00:16:27.240 And many fish species and invertebrate species 324 00:16:27.240 --> 00:16:29.670 use seagrass meadows as nursery habitats 325 00:16:29.670 --> 00:16:31.050 to raise their young. 326 00:16:31.050 --> 00:16:35.010 So seagrasses are really important as a driver 327 00:16:35.010 --> 00:16:37.863 for the next generation of organisms as well. 328 00:16:41.310 --> 00:16:43.770 So unfortunately, like many marine organisms, 329 00:16:43.770 --> 00:16:47.403 seagrasses are under threat due to a number of reasons. 330 00:16:48.300 --> 00:16:50.460 First one is rising ocean temperatures 331 00:16:50.460 --> 00:16:52.230 can cause stress for the seagrass, 332 00:16:52.230 --> 00:16:53.730 which is bad for their health. 333 00:16:54.720 --> 00:16:56.610 There's also human made pollution. 334 00:16:56.610 --> 00:16:59.130 And so agricultural runoff into these areas 335 00:16:59.130 --> 00:17:02.250 can cause algal blooms that decreases the light 336 00:17:02.250 --> 00:17:05.163 that's available to get down to the seagrass plants. 337 00:17:07.170 --> 00:17:12.170 Also, maritime activities can cause the physical removal 338 00:17:12.540 --> 00:17:13.890 of seagrass plants as well. 339 00:17:13.890 --> 00:17:17.790 So a big one is when boats are going through seagrass beds, 340 00:17:17.790 --> 00:17:19.770 their propellers can catch on the plants 341 00:17:19.770 --> 00:17:21.780 and actually rip them up from the roots 342 00:17:21.780 --> 00:17:23.610 and cause these scars that you're seeing 343 00:17:23.610 --> 00:17:25.680 in this bottom picture here. 344 00:17:25.680 --> 00:17:28.200 And also dredging is another activity 345 00:17:28.200 --> 00:17:30.333 that's physically removing these plants. 346 00:17:32.160 --> 00:17:34.710 And then there's also disease, 347 00:17:34.710 --> 00:17:37.263 which I will get into in the next slide. 348 00:17:39.960 --> 00:17:42.480 So I'm really interested in studying seagrasses 349 00:17:42.480 --> 00:17:47.220 as a system that is affected by a seagrass wasting disease. 350 00:17:47.220 --> 00:17:49.080 And so we can go back to our disease triangle 351 00:17:49.080 --> 00:17:50.250 we were talking about earlier 352 00:17:50.250 --> 00:17:52.590 and just try to fill in the different points 353 00:17:52.590 --> 00:17:56.100 with the key players in this particular system. 354 00:17:56.100 --> 00:17:57.540 And so we have our environment, 355 00:17:57.540 --> 00:18:00.030 so it's a waterborne pathogen. 356 00:18:00.030 --> 00:18:01.920 The seagrasses live in water, 357 00:18:01.920 --> 00:18:04.240 and we know that the pathogen itself 358 00:18:05.610 --> 00:18:06.870 is also transmitted 359 00:18:06.870 --> 00:18:09.723 with blade-to-blade contact between plants. 360 00:18:11.070 --> 00:18:14.793 We have our hosts, the temperate seagrass, Zostera marina, 361 00:18:16.380 --> 00:18:18.000 and then we also have our pathogen, 362 00:18:18.000 --> 00:18:22.354 the slime mold protist, Labyrinthula zosterae. 363 00:18:22.354 --> 00:18:24.904 And so like other diseases that we've talked about, 364 00:18:26.070 --> 00:18:29.310 there are visual symptoms of this disease as well. 365 00:18:29.310 --> 00:18:32.430 And so you can see on the picture to the left, 366 00:18:32.430 --> 00:18:36.300 the pathogen actually causes these dark necrotic lesions 367 00:18:36.300 --> 00:18:38.880 on an otherwise green seagrass tissue. 368 00:18:38.880 --> 00:18:42.640 And the pathogen destroys the cell walls of the plant 369 00:18:43.680 --> 00:18:45.927 and gets rid of the chloroplast. 370 00:18:45.927 --> 00:18:48.270 And so it makes it unable for the plant 371 00:18:48.270 --> 00:18:51.630 to conduct efficient photosynthesis. 372 00:18:51.630 --> 00:18:54.210 And over time these lesions can grow 373 00:18:54.210 --> 00:18:56.643 and the plant can eventually die from this. 374 00:18:58.080 --> 00:19:01.050 And so I'm really interested in asking questions 375 00:19:01.050 --> 00:19:03.450 about the pathogen in this disease triangle. 376 00:19:03.450 --> 00:19:07.620 So when I'm looking at my different sites, 377 00:19:07.620 --> 00:19:11.520 how many strains of the pathogen are out there? 378 00:19:11.520 --> 00:19:14.130 Are there areas in the estuary with little disease 379 00:19:14.130 --> 00:19:16.320 versus areas with a lot of disease? 380 00:19:16.320 --> 00:19:19.860 And does this disease intensity vary seasonally? 381 00:19:19.860 --> 00:19:22.290 And so I really want to bring all these questions 382 00:19:22.290 --> 00:19:25.020 back to how can I protect vulnerable populations 383 00:19:25.020 --> 00:19:27.033 of seagrass from this disease? 384 00:19:29.516 --> 00:19:30.780 So to give you a bit more context 385 00:19:30.780 --> 00:19:33.120 of where I'm conducting this research, 386 00:19:33.120 --> 00:19:36.390 I work in two Northern California estuaries, 387 00:19:36.390 --> 00:19:38.220 Bodega Bay and Tomales Bay. 388 00:19:38.220 --> 00:19:40.860 So these are about an hour and a half north 389 00:19:40.860 --> 00:19:42.840 of San Francisco. 390 00:19:42.840 --> 00:19:46.590 And we have years in environmental data at these sites, 391 00:19:46.590 --> 00:19:48.450 and we know that they have seagrass beds 392 00:19:48.450 --> 00:19:53.160 that have been persistent despite changing ocean conditions 393 00:19:53.160 --> 00:19:54.783 in the last decade. 394 00:19:55.860 --> 00:19:59.160 And the interesting thing about Tomales Bay in particular 395 00:19:59.160 --> 00:20:01.620 is that it actually develops a temperature gradient 396 00:20:01.620 --> 00:20:02.453 during the summer. 397 00:20:02.453 --> 00:20:03.780 So these different colors 398 00:20:03.780 --> 00:20:06.303 are showing you different sea surface temperature. 399 00:20:08.010 --> 00:20:11.100 And you can see that that purple blue color 400 00:20:11.100 --> 00:20:12.780 near the map of the estuary, 401 00:20:12.780 --> 00:20:15.150 where it gets input from the ocean, 402 00:20:15.150 --> 00:20:16.890 is a much cooler temperature 403 00:20:16.890 --> 00:20:21.693 than near the head of the estuary in that red orange area. 404 00:20:22.920 --> 00:20:27.300 And so we actually have sites that we keep going back to 405 00:20:27.300 --> 00:20:29.580 to monitor at these yellow stars 406 00:20:29.580 --> 00:20:32.130 that are across this temperature gradient. 407 00:20:32.130 --> 00:20:35.520 And so that really gives us a natural lab to work from 408 00:20:35.520 --> 00:20:38.613 as we try to see what the disease is doing over time. 409 00:20:40.770 --> 00:20:42.930 So how do we actually go about doing this? 410 00:20:42.930 --> 00:20:47.190 So the sites we go to are intertidal seagrass meadows. 411 00:20:47.190 --> 00:20:49.320 So that means that during low tide 412 00:20:49.320 --> 00:20:51.870 they are generally uncovered by water, 413 00:20:51.870 --> 00:20:53.670 much more easily accessible 414 00:20:53.670 --> 00:20:57.783 and easier to count the seagrass as well. 415 00:20:58.680 --> 00:21:01.470 However, the seagrass at our sites also grows in mud flats, 416 00:21:01.470 --> 00:21:04.170 which means that you get a very decent workout 417 00:21:04.170 --> 00:21:05.790 tromping through the mud, 418 00:21:05.790 --> 00:21:09.120 and some sites are easier to get to than others. 419 00:21:09.120 --> 00:21:12.907 We're actually thinking about making a T-shirt being like, 420 00:21:12.907 --> 00:21:15.720 "Oh, you survived (chuckles) the mud," 421 00:21:15.720 --> 00:21:17.793 because it can get quite messy. 422 00:21:19.920 --> 00:21:22.440 So sometimes, you know, even our scientific tools 423 00:21:22.440 --> 00:21:23.730 can become our crutch 424 00:21:23.730 --> 00:21:25.350 when we are trying to get out of the mud, 425 00:21:25.350 --> 00:21:27.900 as you can see from these two pictures here 426 00:21:27.900 --> 00:21:30.363 of me at one of our bodega sites. 427 00:21:33.660 --> 00:21:35.490 And so I'm gonna describe the field like we do 428 00:21:35.490 --> 00:21:38.730 to determine current disease levels out in the field, 429 00:21:38.730 --> 00:21:42.390 and then talk about some of the lab work I'm pairing it with 430 00:21:42.390 --> 00:21:46.410 to learn more about what pathogen is present out there. 431 00:21:46.410 --> 00:21:49.410 So here's a picture of me holding all the gear I use 432 00:21:49.410 --> 00:21:51.180 for my disease surveys. 433 00:21:51.180 --> 00:21:53.220 So we have our transect tapes, 434 00:21:53.220 --> 00:21:55.470 which are essentially giant tape measures we use 435 00:21:55.470 --> 00:21:58.200 to make sure that we're collecting along a straight line 436 00:21:58.200 --> 00:21:59.403 of a certain length. 437 00:22:00.540 --> 00:22:01.800 I'm holding some poles 438 00:22:01.800 --> 00:22:04.500 to anchor those transects into the mud. 439 00:22:04.500 --> 00:22:07.170 Datasheets, of course, to write down our observations 440 00:22:07.170 --> 00:22:08.523 and our measurements. 441 00:22:10.380 --> 00:22:15.060 A quadrat, which is essentially just a square of PVC pipes 442 00:22:15.060 --> 00:22:16.800 that is a standard area. 443 00:22:16.800 --> 00:22:19.170 And so I use that to look at how much seagrass 444 00:22:19.170 --> 00:22:20.673 is out there at my sites. 445 00:22:22.080 --> 00:22:25.650 Collection baggies to bring back samples to the lab. 446 00:22:25.650 --> 00:22:27.990 A GPS to make sure I know where I'm going 447 00:22:27.990 --> 00:22:30.873 and I'm going to the same place every time. 448 00:22:32.143 --> 00:22:33.720 And waders, which despite being 449 00:22:33.720 --> 00:22:35.970 an obvious fashion statement, 450 00:22:35.970 --> 00:22:38.190 are also just these insulated overalls 451 00:22:38.190 --> 00:22:43.050 that help me keep my clothes dry and not muddy, 452 00:22:43.050 --> 00:22:46.773 and then also help me tromp through the mud a bit better. 453 00:22:50.010 --> 00:22:53.700 And so I conduct seasonal sampling at the same six sites, 454 00:22:53.700 --> 00:22:56.223 so three in Bodega Bay, three in Tomales Bay, 455 00:22:57.600 --> 00:23:00.030 to get a sense of how disease is changing 456 00:23:00.030 --> 00:23:01.650 over the span of a year. 457 00:23:01.650 --> 00:23:03.540 So on the picture to the left, 458 00:23:03.540 --> 00:23:08.100 you can see me rolling out that transect on a seagrass bed 459 00:23:08.100 --> 00:23:10.080 at one of our bodega sites. 460 00:23:10.080 --> 00:23:13.050 And the picture to the right is an example of what I observe 461 00:23:13.050 --> 00:23:15.270 when I'm looking at the abundance of seagrass 462 00:23:15.270 --> 00:23:16.650 along my transect. 463 00:23:16.650 --> 00:23:19.560 And so I would actually count all the seagrass chutes 464 00:23:19.560 --> 00:23:21.420 that are within that white square 465 00:23:21.420 --> 00:23:24.300 and then estimate how much of that square is seagrass 466 00:23:24.300 --> 00:23:25.140 versus bare mud. 467 00:23:25.140 --> 00:23:29.970 So I would estimate that this is about maybe 20% seagrass. 468 00:23:29.970 --> 00:23:31.320 And I just wanna emphasize 469 00:23:31.320 --> 00:23:33.090 this is really giving us a snapshot 470 00:23:33.090 --> 00:23:34.980 of the current disease load 471 00:23:34.980 --> 00:23:37.230 in seagrass abundance at that time. 472 00:23:37.230 --> 00:23:39.030 But given enough time points, 473 00:23:39.030 --> 00:23:41.580 we would hopefully be able to paint a more clear picture 474 00:23:41.580 --> 00:23:43.980 of what's happening throughout the year as well. 475 00:23:48.630 --> 00:23:50.280 And so just to give you a sense 476 00:23:50.280 --> 00:23:53.010 of kinda what we're collecting and how we're using it, 477 00:23:53.010 --> 00:23:56.790 we take blades from the seagrass plants, 478 00:23:56.790 --> 00:24:00.150 bring them back to lab, wash them off, measure them, 479 00:24:00.150 --> 00:24:02.460 and then we scan them on sheet transparencies 480 00:24:02.460 --> 00:24:04.590 to document how severe the disease is. 481 00:24:04.590 --> 00:24:08.220 So these two pictures are an example 482 00:24:08.220 --> 00:24:11.310 of those sheet transparencies, 483 00:24:11.310 --> 00:24:13.380 and they're actually from the same time point, 484 00:24:13.380 --> 00:24:14.880 but two different sites. 485 00:24:14.880 --> 00:24:17.310 So you can see the picture on the left. 486 00:24:17.310 --> 00:24:18.660 The seagrass seems really healthy. 487 00:24:18.660 --> 00:24:20.310 It's a vibrant green color. 488 00:24:20.310 --> 00:24:22.920 There are not many lesions on there. 489 00:24:22.920 --> 00:24:25.470 If they are, they're like really small. 490 00:24:25.470 --> 00:24:27.450 However, at the site on the right, 491 00:24:27.450 --> 00:24:30.840 there's these like darker patches of lesions 492 00:24:30.840 --> 00:24:34.380 and it's much more obvious that these are disease plants. 493 00:24:34.380 --> 00:24:36.240 And so this kinda just goes to show 494 00:24:36.240 --> 00:24:39.720 that even at the same point in time, 495 00:24:39.720 --> 00:24:42.180 there are different disease dynamics that are happening 496 00:24:42.180 --> 00:24:44.160 at these different sites. 497 00:24:44.160 --> 00:24:47.070 And we've seen from past disease surveys 498 00:24:47.070 --> 00:24:50.340 that there can also be a lot of variation within sites 499 00:24:50.340 --> 00:24:52.920 in terms of where disease is peaking 500 00:24:52.920 --> 00:24:55.440 during the year, across years. 501 00:24:55.440 --> 00:24:58.740 So the peak of disease might not stay the same 502 00:24:58.740 --> 00:25:01.230 and being necessarily predictable. 503 00:25:01.230 --> 00:25:03.573 And then to analyze this data, 504 00:25:04.560 --> 00:25:06.780 we have collaborators up in Friday Harbor 505 00:25:06.780 --> 00:25:08.550 that utilize an AI technology 506 00:25:08.550 --> 00:25:11.400 to measure the surface area of lesion tissue 507 00:25:11.400 --> 00:25:13.920 versus the surface area of healthy tissue on a blade. 508 00:25:13.920 --> 00:25:16.890 And that can give us a metric of disease severity 509 00:25:16.890 --> 00:25:21.003 that we can then quantify and do statistical tests on. 510 00:25:22.680 --> 00:25:24.600 And so amazing, we have data. 511 00:25:24.600 --> 00:25:27.630 Well, now what? What does this actually tell us? 512 00:25:27.630 --> 00:25:29.700 So it can actually inform us 513 00:25:29.700 --> 00:25:34.563 on what seagrass populations are most vulnerable to disease. 514 00:25:35.400 --> 00:25:36.233 And so you might be asking 515 00:25:36.233 --> 00:25:38.400 what makes a population vulnerable? 516 00:25:38.400 --> 00:25:40.440 And so I know I've definitely experienced this, 517 00:25:40.440 --> 00:25:41.640 and probably some of you too, 518 00:25:41.640 --> 00:25:44.580 where you've had a really crazy week, really hectic, 519 00:25:44.580 --> 00:25:47.700 just running around everywhere, feeling a little rundown, 520 00:25:47.700 --> 00:25:50.760 and it might be easier for you to catch a cold. 521 00:25:50.760 --> 00:25:52.920 And so the same thing actually happens with seagrasses 522 00:25:52.920 --> 00:25:55.710 where if seagrasses are under stress 523 00:25:55.710 --> 00:25:57.870 from either warming temperatures or pollution, 524 00:25:57.870 --> 00:26:01.020 then they're much more susceptible to diseases. 525 00:26:01.020 --> 00:26:01.890 On the flip side, 526 00:26:01.890 --> 00:26:04.080 there also might be populations of seagrasses 527 00:26:04.080 --> 00:26:06.270 that have low levels of disease 528 00:26:06.270 --> 00:26:08.670 and have experienced that for a very long time. 529 00:26:08.670 --> 00:26:10.560 So they've kinda become a little more built up 530 00:26:10.560 --> 00:26:13.170 and resistant to that disease, 531 00:26:13.170 --> 00:26:16.470 and it means that those seagrass beds are more resilient. 532 00:26:16.470 --> 00:26:19.020 So it's really important to identify those as well. 533 00:26:20.310 --> 00:26:22.770 And so the second part of my experiment 534 00:26:22.770 --> 00:26:24.660 is actually delving into the idea 535 00:26:24.660 --> 00:26:26.670 that the pathogen is gonna vary at these sites. 536 00:26:26.670 --> 00:26:28.890 So knowing that disease outbreaks 537 00:26:28.890 --> 00:26:30.510 are going to keep recurring, 538 00:26:30.510 --> 00:26:33.300 how can we manage them better when they do? 539 00:26:33.300 --> 00:26:35.070 And so while we can't vaccinate 540 00:26:35.070 --> 00:26:37.170 every single seagrass plant out there, 541 00:26:37.170 --> 00:26:39.360 there is a distinct lack of information 542 00:26:39.360 --> 00:26:40.980 about the pathogen's ability 543 00:26:40.980 --> 00:26:43.680 to infect different populations. 544 00:26:43.680 --> 00:26:46.440 And so I'm trying to fill this gap in with my lab work, 545 00:26:46.440 --> 00:26:47.823 which I'll talk about next. 546 00:26:51.210 --> 00:26:53.520 All right, so in order to learn more about 547 00:26:53.520 --> 00:26:56.910 if there are differences in the pathogen at my site, 548 00:26:56.910 --> 00:26:58.260 I'm gonna try to culture them 549 00:26:58.260 --> 00:27:00.660 in a controlled environment in lab. 550 00:27:00.660 --> 00:27:03.210 And I like to liken this to taking care of a houseplant. 551 00:27:03.210 --> 00:27:04.230 Like, in many ways, 552 00:27:04.230 --> 00:27:06.660 you know, like a houseplant needs resources 553 00:27:06.660 --> 00:27:09.150 like soil, water, sunlight, 554 00:27:09.150 --> 00:27:12.360 and sometimes even temperature is really important. 555 00:27:12.360 --> 00:27:14.130 And we do a very similar thing, 556 00:27:14.130 --> 00:27:15.810 trying to grow this pathogen in lab. 557 00:27:15.810 --> 00:27:19.680 So we have Petri dishes with seawater serum agar 558 00:27:19.680 --> 00:27:21.240 which contains all the nutrients 559 00:27:21.240 --> 00:27:22.893 the pathogen needs to thrive. 560 00:27:23.730 --> 00:27:27.030 We place lesioned eelgrass tissue on the Petri dish. 561 00:27:27.030 --> 00:27:29.590 And so those darker patches are areas 562 00:27:31.140 --> 00:27:32.700 where the pathogen is active. 563 00:27:32.700 --> 00:27:35.490 And so we're hoping it'll come out of the seagrass tissue 564 00:27:35.490 --> 00:27:38.370 and grow onto this nice agar we set up. 565 00:27:38.370 --> 00:27:41.220 And, you know, after maybe about a week, 566 00:27:41.220 --> 00:27:43.050 we might get something like this. 567 00:27:43.050 --> 00:27:44.730 And so those yellow arrows 568 00:27:44.730 --> 00:27:48.780 are pointing to that kind of like white fuzzy clumps 569 00:27:48.780 --> 00:27:50.940 that you're seeing on the agar dish. 570 00:27:50.940 --> 00:27:54.030 And those are actually areas of very dense pathogen. 571 00:27:54.030 --> 00:27:55.920 So that's exactly what we want. 572 00:27:55.920 --> 00:27:58.170 However, we do wanna make sure, when we're doing this, 573 00:27:58.170 --> 00:28:01.770 that we are only isolating the pathogen that we want. 574 00:28:01.770 --> 00:28:04.620 And so we're gonna take a little square of that agar 575 00:28:04.620 --> 00:28:06.900 and put it onto a plate of new media 576 00:28:06.900 --> 00:28:08.820 and then watch that grow out. 577 00:28:08.820 --> 00:28:13.020 And so the cells that originally started 578 00:28:13.020 --> 00:28:14.430 on that little square of agar 579 00:28:14.430 --> 00:28:17.220 are growing out onto this new media very happily. 580 00:28:17.220 --> 00:28:19.500 And so it's reaching the point 581 00:28:19.500 --> 00:28:21.570 where the culture is kinda that red border 582 00:28:21.570 --> 00:28:22.773 that I've drawn there. 583 00:28:24.180 --> 00:28:26.880 And so this is definitely a learning process. 584 00:28:26.880 --> 00:28:30.870 You can get contamination on a lot of your plates 585 00:28:30.870 --> 00:28:35.790 or yourselves could use up all the media and then die. 586 00:28:35.790 --> 00:28:37.590 And so you really need to be consistent 587 00:28:37.590 --> 00:28:40.380 about checking on the cultures. 588 00:28:40.380 --> 00:28:44.220 And in that way, I think it is very similar 589 00:28:44.220 --> 00:28:45.513 to growing a houseplant. 590 00:28:48.840 --> 00:28:51.240 And so the end goal for my research 591 00:28:51.240 --> 00:28:54.390 is to be able to create a disease risk map 592 00:28:54.390 --> 00:28:56.190 of the estuaries that I work in, 593 00:28:56.190 --> 00:28:58.830 similar to this temperature map of Tomales Bay 594 00:28:58.830 --> 00:29:00.300 shown to the right. 595 00:29:00.300 --> 00:29:01.530 And so with my field work, 596 00:29:01.530 --> 00:29:05.550 I'm looking at how disease intensity 597 00:29:05.550 --> 00:29:09.150 throughout the year varies and what that looks like. 598 00:29:09.150 --> 00:29:11.310 And then with lab work, I'm delving 599 00:29:11.310 --> 00:29:14.430 into the different strains of the pathogen in the area. 600 00:29:14.430 --> 00:29:16.140 And so with these two IDs together, 601 00:29:16.140 --> 00:29:17.400 I'm hoping to combine them 602 00:29:17.400 --> 00:29:20.400 and be able to kind of predict disease hotspots 603 00:29:20.400 --> 00:29:23.550 that are occurring in the estuary. 604 00:29:23.550 --> 00:29:24.870 And then perhaps one question 605 00:29:24.870 --> 00:29:26.550 I might be able to answer from that is, 606 00:29:26.550 --> 00:29:28.320 do these disease levels reflect 607 00:29:28.320 --> 00:29:31.070 kind of the temperature patterns that we also see here? 608 00:29:32.580 --> 00:29:34.920 And through my work 609 00:29:34.920 --> 00:29:37.320 with the Greater Farallones National Marine Sanctuary, 610 00:29:37.320 --> 00:29:40.710 I am also hoping that my work will be useful to managers. 611 00:29:40.710 --> 00:29:42.930 So when managers are deciding, 612 00:29:42.930 --> 00:29:45.780 you know, what areas they wanna take donor plants from 613 00:29:45.780 --> 00:29:50.223 for seagrass restoration projects, 614 00:29:51.120 --> 00:29:52.620 this might be something that they look at 615 00:29:52.620 --> 00:29:55.740 to make sure that they're not taking plants from a site 616 00:29:55.740 --> 00:29:59.790 that has an extremely infectious pathogen around, 617 00:29:59.790 --> 00:30:01.860 as moving those to different parts of the estuary 618 00:30:01.860 --> 00:30:02.910 would be problematic. 619 00:30:03.960 --> 00:30:07.240 And so my work will also 620 00:30:08.100 --> 00:30:10.290 hopefully give us some predictive power 621 00:30:10.290 --> 00:30:13.260 in terms of what areas in the estuary 622 00:30:13.260 --> 00:30:16.560 are gonna be prone to disease now or in the future. 623 00:30:16.560 --> 00:30:20.430 And so identifying both resilient populations 624 00:30:20.430 --> 00:30:25.430 and also vulnerable populations that might be prioritized 625 00:30:26.190 --> 00:30:28.540 when we're thinking about conservation efforts. 626 00:30:30.930 --> 00:30:32.760 And so I just wanna go over this 627 00:30:32.760 --> 00:30:36.390 because I get this question a lot from either managers 628 00:30:36.390 --> 00:30:38.880 or people I run into at my field site. 629 00:30:38.880 --> 00:30:41.300 So obviously kinda the first thought is, 630 00:30:41.300 --> 00:30:42.960 if this disease is so dangerous, 631 00:30:42.960 --> 00:30:44.583 like are we looking for a cure? 632 00:30:45.810 --> 00:30:48.810 And I will say it's a very complicated question. 633 00:30:48.810 --> 00:30:52.950 With marine diseases, everything, you know, is so fluid, 634 00:30:52.950 --> 00:30:54.900 the pathogen is waterborne, 635 00:30:54.900 --> 00:30:59.430 and so it's really hard to put in large scale interventions 636 00:30:59.430 --> 00:31:00.900 without negatively harming 637 00:31:00.900 --> 00:31:03.840 some other organism in the environment. 638 00:31:03.840 --> 00:31:07.230 And with a continuous rise in ocean temperature, 639 00:31:07.230 --> 00:31:10.170 we know that disease outbreaks are increasing 640 00:31:10.170 --> 00:31:13.296 in both occurrence and severity. 641 00:31:13.296 --> 00:31:15.210 But this is not a doom and gloom story. 642 00:31:15.210 --> 00:31:17.190 That doesn't mean that there's nothing we can do 643 00:31:17.190 --> 00:31:19.743 to help these issues. 644 00:31:21.000 --> 00:31:23.250 There's actually some really exciting avenues of research 645 00:31:23.250 --> 00:31:25.860 with microbial communities in seagrasses 646 00:31:25.860 --> 00:31:27.750 and how they might be able to help bolster 647 00:31:27.750 --> 00:31:30.630 the ability of seagrasses to fight off infection. 648 00:31:30.630 --> 00:31:34.260 So this is similar to how you might drink probiotics 649 00:31:34.260 --> 00:31:37.170 to accumulate good bacteria in your body 650 00:31:37.170 --> 00:31:39.690 and help you feel more energized. 651 00:31:39.690 --> 00:31:42.930 And we can also focus on decreasing 652 00:31:42.930 --> 00:31:44.680 other stressors in the environment 653 00:31:45.540 --> 00:31:49.740 such as pollution or boating practices 654 00:31:49.740 --> 00:31:51.450 that are gonna tear up the seagrasses. 655 00:31:51.450 --> 00:31:56.250 So making it as ideal environment 656 00:31:56.250 --> 00:31:57.813 for seagrasses as possible. 657 00:31:59.220 --> 00:32:01.200 And there's also some really awesome 658 00:32:01.200 --> 00:32:03.750 successful restoration stories of seagrasses. 659 00:32:03.750 --> 00:32:07.950 So here in California, down in Southern California, 660 00:32:07.950 --> 00:32:12.000 Morro Bay in 2017, 661 00:32:12.000 --> 00:32:14.580 they had once thriving eelgrass beds 662 00:32:14.580 --> 00:32:18.120 that was actually cut down to about 13 acres, 663 00:32:18.120 --> 00:32:20.850 and then through active restoration efforts 664 00:32:20.850 --> 00:32:23.280 and then also just the eelgrass bed itself 665 00:32:23.280 --> 00:32:28.280 just naturally recovering, they got up to 500 acres in 2021. 666 00:32:28.410 --> 00:32:30.030 And so it's really efforts like this 667 00:32:30.030 --> 00:32:33.270 that showcase how resilient this ecosystem is 668 00:32:33.270 --> 00:32:36.453 and how our efforts do matter and can make a difference. 669 00:32:39.660 --> 00:32:42.600 And so I just wanna wrap up some key takeaways 670 00:32:42.600 --> 00:32:45.423 that I hope you got out of this presentation. 671 00:32:46.440 --> 00:32:50.250 One, that seagrasses are cornerstones for coastal ecosystems 672 00:32:50.250 --> 00:32:53.520 and protect our coastlines and are important to our society 673 00:32:53.520 --> 00:32:55.473 even if we don't really think so. 674 00:32:57.750 --> 00:33:00.120 Two, that by identifying vulnerable 675 00:33:00.120 --> 00:33:03.030 and resilient populations, we can be better prepared 676 00:33:03.030 --> 00:33:05.583 for future seagrass wasting disease outbreaks. 677 00:33:07.110 --> 00:33:10.110 And three, that although marine diseases are common, 678 00:33:10.110 --> 00:33:12.330 they can be managed by decreasing 679 00:33:12.330 --> 00:33:14.193 other stressors in the area. 680 00:33:17.670 --> 00:33:21.360 And so I put together some resources 681 00:33:21.360 --> 00:33:22.680 if you're interested in learning more 682 00:33:22.680 --> 00:33:24.930 about the background of seagrasses, 683 00:33:24.930 --> 00:33:27.450 and then also some educational resources. 684 00:33:27.450 --> 00:33:28.380 So there's some awesome, 685 00:33:28.380 --> 00:33:30.810 like, free teaching tools out there about seagrasses, 686 00:33:30.810 --> 00:33:33.720 this is only, like, a small snapshot of them, 687 00:33:33.720 --> 00:33:35.760 but I have put these in a little handout 688 00:33:35.760 --> 00:33:37.893 that that QR code will take you to. 689 00:33:39.450 --> 00:33:42.090 And so I wanted to just say 690 00:33:42.090 --> 00:33:44.040 thank you so much for joining me today, 691 00:33:45.040 --> 00:33:46.800 and please feel free to email me 692 00:33:46.800 --> 00:33:48.200 with any questions you have. 693 00:33:51.480 --> 00:33:55.320 Oh, thank you so much, Serina, for your great presentation 694 00:33:55.320 --> 00:33:57.870 and we appreciate it. 695 00:33:57.870 --> 00:34:01.380 So Brian and I will look through these questions here. 696 00:34:01.380 --> 00:34:04.350 A few have already come in, but for the rest of you, 697 00:34:04.350 --> 00:34:06.930 go ahead and start thinking about your questions, 698 00:34:06.930 --> 00:34:09.750 getting them typed into the question box. 699 00:34:09.750 --> 00:34:14.670 But to kick it off, when you were talking about 700 00:34:14.670 --> 00:34:18.240 the different salinity and temperature in the two bays, 701 00:34:18.240 --> 00:34:20.250 someone's asking about does Tomales Bay 702 00:34:20.250 --> 00:34:23.310 also have a salinity gradient, 703 00:34:23.310 --> 00:34:28.110 and how do the salinities compare between the two bays? 704 00:34:28.110 --> 00:34:30.600 Mm, yeah, that's a great question. 705 00:34:30.600 --> 00:34:34.260 So I think that, (chuckles) 706 00:34:34.260 --> 00:34:38.370 yeah, so there is a salinity gradient in Tamales Bay, 707 00:34:38.370 --> 00:34:41.010 but it's for such a short period of time 708 00:34:41.010 --> 00:34:43.290 and it's so unpredictable 709 00:34:43.290 --> 00:34:46.027 that I think it's much harder to kind of say that, 710 00:34:46.027 --> 00:34:47.610 "Oh, this is a constant trend," 711 00:34:47.610 --> 00:34:49.650 or, "This is something that we can correlate 712 00:34:49.650 --> 00:34:51.570 with our disease metrics." 713 00:34:51.570 --> 00:34:54.120 So Tomales Bay does have 714 00:34:54.120 --> 00:34:55.860 a couple of different freshwater inputs 715 00:34:55.860 --> 00:34:58.860 from different creeks, and one of our sites is actually 716 00:34:58.860 --> 00:35:00.840 by one of those freshwater inputs. 717 00:35:00.840 --> 00:35:02.820 And so it's really during the winter time 718 00:35:02.820 --> 00:35:05.820 when you get snowmelt coming down or the rainy, 719 00:35:05.820 --> 00:35:07.920 or, yeah, I guess in this case, more the rainy season. 720 00:35:07.920 --> 00:35:09.060 Sorry, I'm from the East Coast, 721 00:35:09.060 --> 00:35:11.643 so winter and snow are kinda synonymous. 722 00:35:13.140 --> 00:35:16.470 And, yeah, so it's really only during maybe, 723 00:35:16.470 --> 00:35:18.390 you know, a couple days or a week 724 00:35:18.390 --> 00:35:20.220 during kinda those rainy winter seasons 725 00:35:20.220 --> 00:35:22.440 that you might actually get a really big influx 726 00:35:22.440 --> 00:35:24.600 of fresh water into the system. 727 00:35:24.600 --> 00:35:28.260 So in that case, there is kind of a very, 728 00:35:28.260 --> 00:35:32.010 I would say, measurable and significant change in salinity, 729 00:35:32.010 --> 00:35:35.340 but because of the current system in the bay, 730 00:35:35.340 --> 00:35:37.020 that, like, water residence 731 00:35:37.020 --> 00:35:39.670 is not necessarily gonna stick there for a long time. 732 00:35:40.590 --> 00:35:43.230 And, yeah, so I think that, 733 00:35:43.230 --> 00:35:45.120 you know, it is definitely possible 734 00:35:45.120 --> 00:35:47.280 that a salinity can have an effect in this case, 735 00:35:47.280 --> 00:35:49.080 but it's not necessarily a system 736 00:35:49.080 --> 00:35:53.550 where it's a big factor right now. 737 00:35:53.550 --> 00:35:54.900 Well, and that kind of a follow-up question, 738 00:35:54.900 --> 00:35:56.610 someone was asking like that, 739 00:35:56.610 --> 00:35:59.910 so salinity doesn't really necessarily play a role 740 00:35:59.910 --> 00:36:03.030 in explaining the wasting disease incidents then. 741 00:36:03.030 --> 00:36:05.580 This was kind of a follow-up question. 742 00:36:05.580 --> 00:36:08.700 So I will say that there have been studies 743 00:36:08.700 --> 00:36:13.700 that have looked at in low salinity environments, 744 00:36:13.710 --> 00:36:16.950 so in environments with a lot of freshwater input, 745 00:36:16.950 --> 00:36:20.790 the pathogen is shown to, you know, not do as well. 746 00:36:20.790 --> 00:36:24.060 And so I will say that there definitely could be an effect, 747 00:36:24.060 --> 00:36:26.340 but I think that for this system in particular, 748 00:36:26.340 --> 00:36:29.040 in Tomales Bay in particular, 749 00:36:29.040 --> 00:36:31.800 it would be much harder to kinda pin down those effects 750 00:36:31.800 --> 00:36:34.350 in terms of it would be a big enough difference in salinity 751 00:36:34.350 --> 00:36:36.210 for a long enough period of time 752 00:36:36.210 --> 00:36:39.153 to actually help us explain these disease variations. 753 00:36:43.170 --> 00:36:45.093 Brian, are you joinin' us? 754 00:36:48.270 --> 00:36:49.950 It's possible we lost him. 755 00:36:49.950 --> 00:36:52.740 We've had some wonky things with our, oh, there he is. 756 00:36:52.740 --> 00:36:53.573 Yay. All right, Brian, 757 00:36:53.573 --> 00:36:56.777 I'll let you take a question here to send to Serina. 758 00:37:01.900 --> 00:37:03.540 There was a question in the chat 759 00:37:03.540 --> 00:37:07.290 that was about how this kind of disease 760 00:37:07.290 --> 00:37:09.780 compares with seagrass beds on the East Coast 761 00:37:09.780 --> 00:37:12.243 and how it relates to manatees as well. 762 00:37:13.260 --> 00:37:14.550 Yeah, that's a great question. 763 00:37:14.550 --> 00:37:16.650 So I'm sure you guys remember, 764 00:37:16.650 --> 00:37:18.420 you know, seagrasses are found globally, 765 00:37:18.420 --> 00:37:20.790 so of course we might not have manatees out here, 766 00:37:20.790 --> 00:37:23.370 but that's definitely true in, I believe, Florida. 767 00:37:23.370 --> 00:37:26.730 And I feel like it's actually really cool 768 00:37:26.730 --> 00:37:28.590 to kind of have this global network 769 00:37:28.590 --> 00:37:31.170 of seagrass ecologists working in different places. 770 00:37:31.170 --> 00:37:33.360 And so there is a big group out in, 771 00:37:33.360 --> 00:37:35.430 I believe, Chesapeake Bay out at VIMS 772 00:37:35.430 --> 00:37:37.650 that are, you know, just one of the groups 773 00:37:37.650 --> 00:37:39.903 looking at seagrasses out on the East Coast. 774 00:37:40.800 --> 00:37:43.500 And so they do have wasting disease out there. 775 00:37:43.500 --> 00:37:46.200 I would say there's very few places where wasting disease 776 00:37:46.200 --> 00:37:47.613 is actually not present. 777 00:37:48.450 --> 00:37:52.170 But in terms of whether it's, like, actually necessarily, 778 00:37:52.170 --> 00:37:55.560 like, harming the eelgrass to the extent 779 00:37:55.560 --> 00:38:00.560 where it's in danger of wiping out an entire meadow 780 00:38:00.840 --> 00:38:02.610 is not necessarily true. 781 00:38:02.610 --> 00:38:05.100 I think even in our California estuaries, 782 00:38:05.100 --> 00:38:06.810 you know, we're monitoring the seagrass, 783 00:38:06.810 --> 00:38:09.360 but the seagrass does come back year after year. 784 00:38:09.360 --> 00:38:12.810 And so, again, kinda pointing towards the resilience 785 00:38:12.810 --> 00:38:15.330 of these ecosystems. 786 00:38:15.330 --> 00:38:18.450 And so I think I would have to actually look at some data 787 00:38:18.450 --> 00:38:19.920 to see, you know, comparatively 788 00:38:19.920 --> 00:38:22.830 like what the disease levels are on the different coasts. 789 00:38:22.830 --> 00:38:23.910 But I think it's also, 790 00:38:23.910 --> 00:38:26.430 there are so many different environmental factors, 791 00:38:26.430 --> 00:38:28.440 you know, Atlantic versus Pacific Coast 792 00:38:28.440 --> 00:38:32.220 and different temperature regimes, like, down in Florida. 793 00:38:32.220 --> 00:38:36.030 And so I think that there's a lot of different factors 794 00:38:36.030 --> 00:38:37.410 that you're gonna have to take into account there 795 00:38:37.410 --> 00:38:40.323 when you're trying to compare across regions especially. 796 00:38:42.720 --> 00:38:44.070 Great. 797 00:38:44.070 --> 00:38:48.570 Someone's suggesting, and you can maybe clarify this, 798 00:38:48.570 --> 00:38:51.210 the seagrass, the Z. marina, 799 00:38:51.210 --> 00:38:54.903 is that an introduced exotic species on the West Coast? 800 00:38:56.040 --> 00:39:00.030 Yeah, so I think, I wanna say all the seagrass, 801 00:39:00.030 --> 00:39:03.060 although I definitely need to brush up on this, 802 00:39:03.060 --> 00:39:06.210 was originated in kinda the Japan area 803 00:39:06.210 --> 00:39:08.910 and then kind of migrated all over the world. 804 00:39:08.910 --> 00:39:12.827 So I believe, you know, it's not necessarily native, 805 00:39:14.700 --> 00:39:17.250 I guess I would say, but there are kind of, 806 00:39:17.250 --> 00:39:20.130 I would say, more invasive species than others. 807 00:39:20.130 --> 00:39:21.630 I know Zostera japonica, 808 00:39:21.630 --> 00:39:24.450 which is found a little bit further up the West Coast, 809 00:39:24.450 --> 00:39:26.910 kind of in the Oregon, Washington region 810 00:39:26.910 --> 00:39:28.740 I think it's a lot more common, 811 00:39:28.740 --> 00:39:30.270 I would say that is kind of viewed 812 00:39:30.270 --> 00:39:32.853 as a more invasive seagrass, if you will. 813 00:39:34.170 --> 00:39:36.033 And yeah, I think, again, 814 00:39:37.498 --> 00:39:39.498 I don't have the best knowledge on this, 815 00:39:41.460 --> 00:39:43.593 but that's the best I could explain it. 816 00:39:45.120 --> 00:39:46.650 Oh yeah, we might be asking questions 817 00:39:46.650 --> 00:39:49.563 that are outside your purview, so just let us know. 818 00:39:56.820 --> 00:39:59.640 All right, there's one here. 819 00:39:59.640 --> 00:40:02.640 Like, do you have any data on the amount of carbon 820 00:40:02.640 --> 00:40:05.400 that is stored by all of the seagrasses? 821 00:40:05.400 --> 00:40:08.100 I know we talked about blue carbon ecosystems, 822 00:40:08.100 --> 00:40:11.400 but is that something at your fingertips? 823 00:40:11.400 --> 00:40:15.540 That is not something I know off the top of my head. 824 00:40:15.540 --> 00:40:17.100 I would definitely encourage you guys 825 00:40:17.100 --> 00:40:22.100 to, you know, like Google any resources or papers 826 00:40:24.090 --> 00:40:26.160 'cause I'm sure, you know, that number 827 00:40:26.160 --> 00:40:27.060 is definitely out there. 828 00:40:27.060 --> 00:40:28.890 And I definitely don't wanna tell you a wrong number, 829 00:40:28.890 --> 00:40:32.640 but it's significant enough that people are, 830 00:40:32.640 --> 00:40:34.983 you know, noting it as a blue carbon system. 831 00:40:41.130 --> 00:40:43.620 Go ahead, Brian, if you found another good question here. 832 00:40:43.620 --> 00:40:47.010 There's quite a few, so we're kinda going through it. 833 00:40:47.010 --> 00:40:49.363 Yeah, I'm trying to keep up with all of them. 834 00:40:50.670 --> 00:40:51.633 Let's see. 835 00:40:52.830 --> 00:40:55.800 How long and frequently are you doing your field monitoring 836 00:40:55.800 --> 00:40:59.100 to help make your maps and temperature predictions? 837 00:40:59.100 --> 00:41:01.170 Yeah, so it's a little tricky 838 00:41:01.170 --> 00:41:02.820 because we do intertidal field work. 839 00:41:02.820 --> 00:41:07.820 So, like, relying on the tides gets a little challenging, 840 00:41:07.890 --> 00:41:10.590 especially in the kinda fall/winter area. 841 00:41:10.590 --> 00:41:13.740 So the tides are either super late at night 842 00:41:13.740 --> 00:41:15.480 or super early in the morning. 843 00:41:15.480 --> 00:41:16.920 And then often tides, 844 00:41:16.920 --> 00:41:18.720 they're like not necessarily good tides, 845 00:41:18.720 --> 00:41:21.990 so the water's still pretty deep even at low tide, 846 00:41:21.990 --> 00:41:24.270 that it might make it really difficult for us to go out. 847 00:41:24.270 --> 00:41:29.270 And so I completed almost one year of sampling, 848 00:41:30.630 --> 00:41:35.630 and so I did kind of a seasonal time point in the fall 849 00:41:35.700 --> 00:41:37.650 and a seasonal time point in the winter. 850 00:41:37.650 --> 00:41:41.280 And then from, I guess, February onward 851 00:41:41.280 --> 00:41:43.260 until probably August or September, 852 00:41:43.260 --> 00:41:45.450 I'll be doing monthly sampling time points. 853 00:41:45.450 --> 00:41:48.750 And so we're really hoping to be able to capture 854 00:41:48.750 --> 00:41:51.690 the start of when disease is really starting to ramp up 855 00:41:51.690 --> 00:41:55.260 in these seagrass beds, which might be around 856 00:41:55.260 --> 00:41:56.940 kind of like the spring-ish time point 857 00:41:56.940 --> 00:41:59.730 'cause then we do see a lot of disease lesions 858 00:41:59.730 --> 00:42:01.650 by the time you get to June, July, August. 859 00:42:01.650 --> 00:42:03.840 So we're hoping to be able to cover 860 00:42:03.840 --> 00:42:05.310 kind of like a breadth of area 861 00:42:05.310 --> 00:42:07.230 and then we'll see if that's necessary 862 00:42:07.230 --> 00:42:11.523 to continue this temporally for the next couple of years. 863 00:42:12.900 --> 00:42:15.690 Yeah, so with your analyzing your field data 864 00:42:15.690 --> 00:42:19.530 to determine hotspots, like I'm not sure if I caught this, 865 00:42:19.530 --> 00:42:22.113 can you do that through modeling as well? 866 00:42:23.490 --> 00:42:26.490 Yeah. Yes, exactly. 867 00:42:26.490 --> 00:42:30.360 So there's actually a postdoc in my lab 868 00:42:30.360 --> 00:42:33.480 who is starting to do a project also 869 00:42:33.480 --> 00:42:35.070 on seagrass wasting disease, 870 00:42:35.070 --> 00:42:38.380 and she's developing a model that will be able to 871 00:42:39.240 --> 00:42:41.970 essentially have an output that's very similar to this. 872 00:42:41.970 --> 00:42:44.790 So we are looking at, so for a model, 873 00:42:44.790 --> 00:42:46.530 it would probably need to be some type of, 874 00:42:46.530 --> 00:42:50.160 you know a transmission rate between healthy and infected, 875 00:42:50.160 --> 00:42:54.930 you know a recovery rate from if a plant is infected, 876 00:42:54.930 --> 00:42:57.390 can it recover and like what percentage of plants 877 00:42:57.390 --> 00:43:01.380 are gonna recover, what dosage of, 878 00:43:01.380 --> 00:43:05.100 you know, how many Labyrinthula zosterae cells are necessary 879 00:43:05.100 --> 00:43:06.480 to actually start an infection. 880 00:43:06.480 --> 00:43:09.480 And so there's a lot of smaller, I think, pilot experiments 881 00:43:09.480 --> 00:43:10.830 that need to be done before that 882 00:43:10.830 --> 00:43:12.930 to get information that you then put into a model, 883 00:43:12.930 --> 00:43:15.060 but that is kinda the idea 884 00:43:15.060 --> 00:43:18.810 of, like, using some type of model and technology like that 885 00:43:18.810 --> 00:43:20.410 to be able to create these maps. 886 00:43:26.490 --> 00:43:27.323 There's a couple of questions 887 00:43:27.323 --> 00:43:30.180 that I think kind of connect to each other 888 00:43:30.180 --> 00:43:33.180 regarding kinda genetic sequencing 889 00:43:33.180 --> 00:43:35.610 to determine if there is sort of a resilience. 890 00:43:35.610 --> 00:43:37.500 And then a second question, 891 00:43:37.500 --> 00:43:40.410 if there's the potential for the seagrasses 892 00:43:40.410 --> 00:43:42.360 to build some sort of immunity as well. 893 00:43:43.650 --> 00:43:48.650 Yeah, so it was genetic testing to determine, 894 00:43:48.720 --> 00:43:49.620 what was it again? 895 00:43:52.693 --> 00:43:53.700 A resilience. 896 00:43:53.700 --> 00:43:55.173 Resilience, okay, yes. 897 00:43:56.190 --> 00:44:00.780 Yeah, so I think that I was kind of thinking more, 898 00:44:00.780 --> 00:44:02.160 so there is definitely 899 00:44:02.160 --> 00:44:04.263 a lot of seagrass genetics work going on, 900 00:44:05.190 --> 00:44:08.493 and especially in terms of there are, 901 00:44:10.350 --> 00:44:12.300 yeah, kind of like different genotypes, 902 00:44:12.300 --> 00:44:14.820 and I know there's actually another lab at UC Davis 903 00:44:14.820 --> 00:44:16.500 who's kinda focusing on that 904 00:44:16.500 --> 00:44:19.710 and have looked at seagrass disease metrics as well 905 00:44:19.710 --> 00:44:21.450 in their experiments. 906 00:44:21.450 --> 00:44:23.730 I think for me, I was thinking again, 907 00:44:23.730 --> 00:44:27.330 kind of more about the different pathogen strains 908 00:44:27.330 --> 00:44:28.320 that might be out there. 909 00:44:28.320 --> 00:44:30.840 And so I would sequence those samples 910 00:44:30.840 --> 00:44:32.760 in terms of trying to figure out 911 00:44:32.760 --> 00:44:35.580 are there genetic differences between my pathogen strains? 912 00:44:35.580 --> 00:44:39.180 If so, does that point towards certain factors 913 00:44:39.180 --> 00:44:44.180 that might affect how virulent the pathogen is, 914 00:44:44.610 --> 00:44:47.340 how much it's able to infect a plant. 915 00:44:47.340 --> 00:44:51.420 So I guess I'm kinda going less on the host side of things 916 00:44:51.420 --> 00:44:53.400 because I feel like there are a lot of people doing that 917 00:44:53.400 --> 00:44:55.770 and kinda more on the pathogen side of things 918 00:44:55.770 --> 00:44:57.873 that are a bit more unknown. 919 00:44:58.980 --> 00:45:01.470 And so I think that both those factors 920 00:45:01.470 --> 00:45:04.740 can kinda tell us about resilience 921 00:45:04.740 --> 00:45:07.173 as kinda like a community or population. 922 00:45:08.670 --> 00:45:12.603 And did I forget one other part of your question? 923 00:45:16.320 --> 00:45:17.520 Yeah, I think we forgot too. 924 00:45:17.520 --> 00:45:20.331 What was it, Brian? (laughs) 925 00:45:20.331 --> 00:45:22.302 The immunity and- Oh, immunity. 926 00:45:22.302 --> 00:45:23.998 Yeah. Yes. 927 00:45:23.998 --> 00:45:25.388 Immunity against the pathogen, yeah. 928 00:45:25.388 --> 00:45:26.221 Right, yeah. 929 00:45:26.221 --> 00:45:29.520 So unlike humans, seagrasses do not have 930 00:45:29.520 --> 00:45:30.750 an adaptive immune system. 931 00:45:30.750 --> 00:45:33.217 So they really only have kinda this like innate response to, 932 00:45:33.217 --> 00:45:36.521 "Something's wrong, let's, like, try to fix this." 933 00:45:36.521 --> 00:45:39.840 And so there has been some work done on phenology. 934 00:45:39.840 --> 00:45:41.070 And so these are compounds 935 00:45:41.070 --> 00:45:42.630 that the seagrass is gonna produce 936 00:45:42.630 --> 00:45:45.483 to try to protect itself from any type of threat. 937 00:45:46.650 --> 00:45:48.870 Like, these are mostly chemical compounds. 938 00:45:48.870 --> 00:45:52.323 And so I think that over time, 939 00:45:53.310 --> 00:45:55.440 through different generations, 940 00:45:55.440 --> 00:45:59.610 there might be some indication that seagrasses, 941 00:45:59.610 --> 00:46:02.103 you know, might be able to, 942 00:46:03.270 --> 00:46:05.670 for instance, if your parents, like, had the disease, 943 00:46:05.670 --> 00:46:09.030 like it is possible that maybe that somehow passes down 944 00:46:09.030 --> 00:46:10.023 to the offspring. 945 00:46:11.160 --> 00:46:13.740 And then also kind of within the seagrass plant's life, 946 00:46:13.740 --> 00:46:15.420 like if it's been infected before, 947 00:46:15.420 --> 00:46:18.600 like what's the possibility that it is more, 948 00:46:18.600 --> 00:46:20.190 you know, resistant to that infection? 949 00:46:20.190 --> 00:46:23.400 And I think, like, there's a lot of kinda like hypotheses 950 00:46:23.400 --> 00:46:24.780 that this might happen, 951 00:46:24.780 --> 00:46:28.257 and hopefully some of my work will be able to get at this, 952 00:46:28.257 --> 00:46:30.420 but I do think that's kinda like a burning question 953 00:46:30.420 --> 00:46:31.570 a little bit right now. 954 00:46:34.170 --> 00:46:37.350 This is one specific to your lab work. 955 00:46:37.350 --> 00:46:39.630 So at what temperature, or temperatures, 956 00:46:39.630 --> 00:46:43.230 are you incubating your pathogen isolates in the lab, 957 00:46:43.230 --> 00:46:45.810 and is there a temperature optimum 958 00:46:45.810 --> 00:46:49.800 for these microbes for rapid multiplication? 959 00:46:49.800 --> 00:46:52.680 Mm, yeah, that's a great question. 960 00:46:52.680 --> 00:46:54.630 So I'm actually a little bit lucky 961 00:46:54.630 --> 00:46:58.080 that I kinda stumbled upon the seagrass system in that one, 962 00:46:58.080 --> 00:47:00.900 the pathogen is culturable in lab, which is great, 963 00:47:00.900 --> 00:47:03.000 and that you can just leave the plates 964 00:47:03.000 --> 00:47:06.690 out at room temperature and they seem to grow just fine. 965 00:47:06.690 --> 00:47:08.520 And so I did experiment a little bit 966 00:47:08.520 --> 00:47:12.510 with like raising the temperature 967 00:47:12.510 --> 00:47:16.590 maybe to like 20 degrees Celsius or something like that, 968 00:47:16.590 --> 00:47:18.363 or a little bit higher, like 21, 969 00:47:19.230 --> 00:47:21.930 and it didn't really seem to make that much of a difference. 970 00:47:21.930 --> 00:47:24.210 This is also an area of kinda like 971 00:47:24.210 --> 00:47:26.100 active investigation in my lab 972 00:47:26.100 --> 00:47:27.720 that probably in the next, like, couple weeks 973 00:47:27.720 --> 00:47:30.020 or couple months, like I'm definitely gonna be 974 00:47:30.990 --> 00:47:32.880 getting a lot more into this 975 00:47:32.880 --> 00:47:35.160 in terms of what is the optimum temperature. 976 00:47:35.160 --> 00:47:38.880 And the postdoc in my lab who's working on models 977 00:47:38.880 --> 00:47:40.890 is also working on thermal performance curves 978 00:47:40.890 --> 00:47:42.930 of Labyrinthula zosterae. 979 00:47:42.930 --> 00:47:45.960 So she's also gonna be, you know, looking, 980 00:47:45.960 --> 00:47:47.490 especially at this question of 981 00:47:47.490 --> 00:47:49.470 what is the optimum temperature 982 00:47:49.470 --> 00:47:51.270 that this pathogen can go really well at 983 00:47:51.270 --> 00:47:53.970 and how does that compare to environmental conditions? 984 00:47:58.800 --> 00:48:00.900 There's a few questions as well 985 00:48:00.900 --> 00:48:05.900 about what was the native species before the Z. marina 986 00:48:05.940 --> 00:48:09.630 and then how kind of the ecosystems that they support 987 00:48:09.630 --> 00:48:12.120 and the roles that the two different species have 988 00:48:12.120 --> 00:48:13.670 kind of compared to each other. 989 00:48:14.520 --> 00:48:18.570 So yeah, I feel like as these questions are rolling in 990 00:48:18.570 --> 00:48:19.920 about kinda like native species, 991 00:48:19.920 --> 00:48:23.040 I'm like, "Oh, this is something I should learn more about." 992 00:48:23.040 --> 00:48:25.710 So my sense is that, like, the, 993 00:48:25.710 --> 00:48:28.830 I guess like transmigration of seagrasses from Japan 994 00:48:28.830 --> 00:48:30.660 happened a very long time ago. 995 00:48:30.660 --> 00:48:32.458 So in terms of like nativity, 996 00:48:32.458 --> 00:48:34.860 like what is the time frame that we're thinking about 997 00:48:34.860 --> 00:48:37.050 might be a good question to ponder. 998 00:48:37.050 --> 00:48:41.790 And so I know that, like, seagrasses have been in the area 999 00:48:41.790 --> 00:48:44.913 for, I would say, a pretty substantial amount of time, 1000 00:48:45.870 --> 00:48:49.560 and I don't necessarily, I believe that Zostera marina 1001 00:48:49.560 --> 00:48:54.150 is the majority species that we have here at least 1002 00:48:54.150 --> 00:48:56.163 and has been for a very long time. 1003 00:48:58.340 --> 00:48:59.760 Like, you know, Indigenous tribes 1004 00:48:59.760 --> 00:49:04.233 have been using seagrasses for decades, centuries, 1005 00:49:06.859 --> 00:49:09.390 you know, as a weaving material for baskets 1006 00:49:09.390 --> 00:49:13.320 or as housing material to, like, patch roofs. 1007 00:49:13.320 --> 00:49:15.810 So I would say that it's been here 1008 00:49:15.810 --> 00:49:16.800 a long enough amount of time 1009 00:49:16.800 --> 00:49:19.500 that it has kind of integrated itself into the community 1010 00:49:19.500 --> 00:49:21.000 and how the community uses it. 1011 00:49:21.960 --> 00:49:24.420 And as far as I know, Zostera marina, 1012 00:49:24.420 --> 00:49:27.120 that has been the species that's been present 1013 00:49:27.120 --> 00:49:27.953 for the longest. 1014 00:49:27.953 --> 00:49:32.640 But, again, this is pushing the edges of my knowledge 1015 00:49:32.640 --> 00:49:33.543 a little bit here. 1016 00:49:35.310 --> 00:49:36.960 And this is kind of an interesting question. 1017 00:49:36.960 --> 00:49:39.970 Like, are there other things that can cause 1018 00:49:41.460 --> 00:49:44.070 something that looks similar to the disease lesions, 1019 00:49:44.070 --> 00:49:46.020 like feeding by snails? 1020 00:49:46.020 --> 00:49:49.290 And if so, like, how can you tell the difference? 1021 00:49:49.290 --> 00:49:50.550 Yeah, that's a great question. 1022 00:49:50.550 --> 00:49:54.330 Actually I realized I, like, didn't really get into this. 1023 00:49:54.330 --> 00:49:56.040 But while we're doing our disease surveys, 1024 00:49:56.040 --> 00:50:00.240 we are also documenting grazing scars on the eelgrass. 1025 00:50:00.240 --> 00:50:03.000 So sometimes you'll have little chomps sticking out of it, 1026 00:50:03.000 --> 00:50:04.770 and you're like, "Okay, that's obviously 1027 00:50:04.770 --> 00:50:06.240 like an invertebrate feeding on it," 1028 00:50:06.240 --> 00:50:09.150 or snails in particular will actually scrape away 1029 00:50:09.150 --> 00:50:09.990 the eelgrass tissue. 1030 00:50:09.990 --> 00:50:11.520 So it's actually a little bit translucent 1031 00:50:11.520 --> 00:50:13.830 when you hold it up to the light. 1032 00:50:13.830 --> 00:50:15.990 And so there are definitely indications 1033 00:50:15.990 --> 00:50:19.740 that, you know, like grazing that can happen. 1034 00:50:19.740 --> 00:50:23.340 And there is also bleaching. So seagrass can bleach. 1035 00:50:23.340 --> 00:50:25.650 I think it's maybe less common at our sites. 1036 00:50:25.650 --> 00:50:27.900 I know it's probably more common. 1037 00:50:27.900 --> 00:50:31.440 I've seen it a lot up in the Friday Harbor area 1038 00:50:31.440 --> 00:50:33.990 where the seagrass will actually just turn 1039 00:50:33.990 --> 00:50:36.603 kind of like a translucent light brownish. 1040 00:50:37.440 --> 00:50:39.030 And that's really just because, 1041 00:50:39.030 --> 00:50:41.310 you know, in intertidal environments, 1042 00:50:41.310 --> 00:50:43.260 you're gonna be exposed to the air, to the sun 1043 00:50:43.260 --> 00:50:45.630 for, you know, a couple hours a day. 1044 00:50:45.630 --> 00:50:47.010 And if the sun's really intense, 1045 00:50:47.010 --> 00:50:49.080 then you might end up bleaching. 1046 00:50:49.080 --> 00:50:51.600 So it is, especially I think now 1047 00:50:51.600 --> 00:50:54.780 when a disease hasn't quite ramped up yet, 1048 00:50:54.780 --> 00:50:57.660 there's been a lot of judgment calls we've had to make on, 1049 00:50:57.660 --> 00:51:01.560 is this a lesion or is this, you know, like something else? 1050 00:51:01.560 --> 00:51:03.060 And there's definitely the possibility 1051 00:51:03.060 --> 00:51:05.760 that, you know, there are other pathogens out there 1052 00:51:05.760 --> 00:51:07.620 that are infecting seagrasses 1053 00:51:07.620 --> 00:51:11.730 and might cause similar, like, visual symptoms. 1054 00:51:11.730 --> 00:51:16.350 But yeah, so definitely, definitely true 1055 00:51:16.350 --> 00:51:18.963 that there are other things out there as well. 1056 00:51:21.270 --> 00:51:23.220 All right, Brian, let's get one last question in 1057 00:51:23.220 --> 00:51:25.110 and then we'll do our wrap up slides 1058 00:51:25.110 --> 00:51:26.820 and then Serina's off the hook. 1059 00:51:26.820 --> 00:51:29.700 Lots of great questions coming through. 1060 00:51:29.700 --> 00:51:31.650 Yeah, let's see. 1061 00:51:31.650 --> 00:51:34.410 Do you see any seasonality of outbreaks 1062 00:51:34.410 --> 00:51:36.993 or increase in diseased plants? 1063 00:51:38.310 --> 00:51:40.380 Yes, so we definitely do. 1064 00:51:40.380 --> 00:51:43.620 So temperature is, I would say, 1065 00:51:43.620 --> 00:51:46.680 you know, one of the most obvious driving factors 1066 00:51:46.680 --> 00:51:47.880 in seagrass wasting disease. 1067 00:51:47.880 --> 00:51:50.400 So we definitely see kinda the prime time for disease 1068 00:51:50.400 --> 00:51:51.780 is gonna be that summer period. 1069 00:51:51.780 --> 00:51:54.360 So probably like June to August 1070 00:51:54.360 --> 00:51:56.970 is when you see a ton of disease lesions out there. 1071 00:51:56.970 --> 00:51:58.410 They're very big lesions. 1072 00:51:58.410 --> 00:52:00.513 They're, like, very obviously lesions. 1073 00:52:01.410 --> 00:52:03.720 And so there have been a lot of studies 1074 00:52:03.720 --> 00:52:05.700 kind of identifying that, 1075 00:52:05.700 --> 00:52:07.230 you know, rising ocean temperatures, 1076 00:52:07.230 --> 00:52:08.550 it puts a lot of stress on the plant. 1077 00:52:08.550 --> 00:52:11.760 And then it, you know, possibly also in general 1078 00:52:11.760 --> 00:52:13.530 has been increasing disease outbreaks, 1079 00:52:13.530 --> 00:52:15.120 not just for seagrass wasting disease, 1080 00:52:15.120 --> 00:52:17.340 but for other diseases as well. 1081 00:52:17.340 --> 00:52:20.580 And so, however, I think it's like, 1082 00:52:20.580 --> 00:52:23.580 you know, not quite a closed door answer 1083 00:52:23.580 --> 00:52:26.520 in terms of it's not just rising temperatures 1084 00:52:26.520 --> 00:52:29.070 that are affecting whether an outbreak happens or not. 1085 00:52:29.070 --> 00:52:31.980 There's probably, you know, a myriad of different factors 1086 00:52:31.980 --> 00:52:35.970 that are involved, and so it's kinda hard 1087 00:52:35.970 --> 00:52:37.417 to, like, parse through, you know, like, 1088 00:52:37.417 --> 00:52:39.810 "This is the cause," or like, "This is the reason," 1089 00:52:39.810 --> 00:52:42.723 but we definitely do see a lot more disease in the summer. 1090 00:52:45.900 --> 00:52:46.733 Excellent. 1091 00:52:46.733 --> 00:52:49.800 Well, thank you so much for your great presentation. 1092 00:52:49.800 --> 00:52:52.710 There's been lots of kudos, 1093 00:52:52.710 --> 00:52:56.460 let me get back here to my slides, coming through. 1094 00:52:56.460 --> 00:52:58.500 So we appreciate your time, Serina. 1095 00:52:58.500 --> 00:53:02.160 It's lovely to have one of our Dr. Nancy Foster scholars 1096 00:53:02.160 --> 00:53:05.337 who's a PhD candidate presenting your research 1097 00:53:05.337 --> 00:53:09.600 and what you're finding about seagrass wasting syndrome. 1098 00:53:09.600 --> 00:53:13.170 So I wanted to just wrap up with a few slides here. 1099 00:53:13.170 --> 00:53:14.820 Don't worry about the long URL. 1100 00:53:14.820 --> 00:53:16.650 We're a typical government, everything's long, 1101 00:53:16.650 --> 00:53:18.600 the names of things and URLs, 1102 00:53:18.600 --> 00:53:20.970 but I just popped it into the chat box. 1103 00:53:20.970 --> 00:53:23.460 So an archive of today's presentation 1104 00:53:23.460 --> 00:53:27.540 will be available online within about a week 1105 00:53:27.540 --> 00:53:30.990 for you to share with colleagues or of course, 1106 00:53:30.990 --> 00:53:33.060 for those that didn't make the live presentation, 1107 00:53:33.060 --> 00:53:35.013 they'll be able to see the recording. 1108 00:53:36.240 --> 00:53:40.350 All attendees will also receive a Certificate of Attendance 1109 00:53:40.350 --> 00:53:44.220 for one contact hour of professional development. 1110 00:53:44.220 --> 00:53:46.440 So for those that are watching the recording, 1111 00:53:46.440 --> 00:53:48.180 this is where you can send an email 1112 00:53:48.180 --> 00:53:52.230 to sanctuary.education@noaa.gov 1113 00:53:52.230 --> 00:53:54.900 to ask for your Certificate of Attendance. 1114 00:53:54.900 --> 00:53:56.700 But the rest of you that are attending live, 1115 00:53:56.700 --> 00:53:58.653 this will be emailed to you tomorrow. 1116 00:53:59.730 --> 00:54:02.400 We have a great presentation coming up 1117 00:54:02.400 --> 00:54:05.970 by Dr. Julia Parrish, What's Washed In: 1118 00:54:05.970 --> 00:54:09.660 Seabirds, Marine Debris, and Citizen Science. 1119 00:54:09.660 --> 00:54:12.870 So that's gonna be occurring April 23rd. 1120 00:54:12.870 --> 00:54:15.933 You can go to our webpage to sign up. 1121 00:54:16.830 --> 00:54:18.870 And as always, 1122 00:54:18.870 --> 00:54:22.530 we've been doing this webinar series since 2016 1123 00:54:22.530 --> 00:54:25.320 and we greatly value your feedback. 1124 00:54:25.320 --> 00:54:29.130 So there's a four or five question evaluation 1125 00:54:29.130 --> 00:54:32.490 that wraps up when you close out of GoToWebinar. 1126 00:54:32.490 --> 00:54:35.550 If you wouldn't mind taking literally one 1127 00:54:35.550 --> 00:54:38.100 to one and a half minutes to complete that evaluation, 1128 00:54:38.100 --> 00:54:40.110 we would greatly appreciate it. 1129 00:54:40.110 --> 00:54:42.630 If you're a formal and informal educator, 1130 00:54:42.630 --> 00:54:46.290 there's a longer STEM multimedia distance learning 1131 00:54:46.290 --> 00:54:49.830 evaluation that I'll send in a follow-up email. 1132 00:54:49.830 --> 00:54:53.070 So with that, again, we thank you so much 1133 00:54:53.070 --> 00:54:55.950 for your attendance today. 1134 00:54:55.950 --> 00:54:58.800 We thank Serina for your great presentation, 1135 00:54:58.800 --> 00:55:02.220 Brian for joining me as webinar co-host. 1136 00:55:02.220 --> 00:55:05.880 And with that, it'll conclude today's live presentation. 1137 00:55:05.880 --> 00:55:07.713 Thank you so much. Take care.